Ultrasound activation of TiO₂ in melanoma tumors

Sonodynamic therapy (SDT) is a new modality using ultrasound (US) to activate certain chemical sensitizers for cancer therapy. In this study, the effect of US combined with a nanoparticle titanium dioxide (TiO₂) on melanoma cell was investigated in vitro and in vivo. Melanoma cells (C32) were irradi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of controlled release 2011-01, Vol.149 (2), p.190-195
Main Authors: Harada, Yoshimi, Ogawa, Koichi, Irie, Yutaka, Endo, Hitomi, Feril, Loreto B., Jr, Uemura, Tetsuji, Tachibana, Katsuro
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Apoptosis - radiation effects
Cell Line, Tumor
Cell Survival - drug effects
Cell Survival - radiation effects
cell viability
irradiation
melanoma
Melanoma, Experimental - therapy
Melanoma, Experimental - ultrastructure
Mice
Mice, Inbred BALB C
Mice, Nude
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
nanoparticles
Nanoparticles - administration & dosage
Radiation-Sensitizing Agents - administration & dosage
Radiation-Sensitizing Agents - pharmacology
Radiation-Sensitizing Agents - radiation effects
therapeutics
Titanium - administration & dosage
Titanium - pharmacology
Titanium - radiation effects
titanium dioxide
Ultrasonic Therapy - methods
ultrasonics
Xenograft Model Antitumor Assays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sonodynamic therapy (SDT) is a new modality using ultrasound (US) to activate certain chemical sensitizers for cancer therapy. In this study, the effect of US combined with a nanoparticle titanium dioxide (TiO₂) on melanoma cell was investigated in vitro and in vivo. Melanoma cells (C32) were irradiated with US in the presence and/or absence of TiO₂. Cell viability was measured immediately after US irradiation (1MHz, 0.5 and 1.0W/cm² for 10s). The effect of the combination of TiO₂ and US exposure (1MHz, 1.0W/cm², 2min duration) on subcutaneously implanted C32 solid tumors in mice were investigated by measuring tumor volume regression. The cell viability was significantly decreased only after US irradiation in the presence of TiO₂. In vivo results showed significant inhibition of tumor growth in groups treated with TiO₂ and US. To our knowledge, this is the first report to demonstrate the cell killing effect of TiO₂ nanoparticles under the irradiation US in vitro and in vivo.
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2010.10.012