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The Conversion of Acetoacetate to Pyruvaldehyde

The oxidative decarboxylation of acetoacetate to pyruvaldehyde is catalyzed by sheep heart extracts in the presence of O 2 and Mn 2+ . The product was identified by spectral and chromatographic methods. From fractionation and inhibitor characteristics and the similarity of the catalytic activity to...

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Bibliographic Details
Published in:The Journal of biological chemistry 1967-03, Vol.242 (6), p.1095-1101
Main Authors: Milligan, L P, Baldwin, R L
Format: Article
Language:English
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Summary:The oxidative decarboxylation of acetoacetate to pyruvaldehyde is catalyzed by sheep heart extracts in the presence of O 2 and Mn 2+ . The product was identified by spectral and chromatographic methods. From fractionation and inhibitor characteristics and the similarity of the catalytic activity to that of commercial myoglobin, it is concluded that the catalyst for the conversion in sheep heart extracts is myoglobin. A free radical mechanism which would account for the characteristics and requirements of the reaction is presented.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)96149-1