Exchange of Bound Tritium between DNA and Water at Different Temperatures

A CLUE to the structure of macromolecules in solution is given by the exchange of protons between the macromolecules and the surrounding solvent. We have investigated the exchange of tritium between DNA and water at different temperatures. We have used the technique of ‘Sephadex’ chromatography desc...

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Bibliographic Details
Published in:Nature (London) 1970-03, Vol.225 (5237), p.1043-1045
Main Authors: BIRD, ROBERT E, LARK, KARL G, CURNUTTE, BASIL, MAXFIELD, JOHN E
Format: Article
Language:English
Subjects:
DNA
Humanities and Social Sciences
Ion Exchange
letter
Models, Chemical
multidisciplinary
Science
Science (multidisciplinary)
Thermodynamics
Tritium
Water
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Summary:A CLUE to the structure of macromolecules in solution is given by the exchange of protons between the macromolecules and the surrounding solvent. We have investigated the exchange of tritium between DNA and water at different temperatures. We have used the technique of ‘Sephadex’ chromatography described by Englander 1 and used by Printz and von Hippel 2 to study tritium exchange between DNA and water.
ISSN:0028-0836
1476-4687
DOI:10.1038/2251043a0