Release of Lipopolysaccharide in Escherichia coli Resistant to the Permeability Increase Induced by Ethylenediaminetetraacetate

Mutants of Escherichia coli that fail to show the characteristic increase in permeability induced by brief treatment with ethylenediaminetetraacetate have been isolated. They were examined to see whether they release lipopolysaccharide as expected during such a treatment. Mutants derived from two st...

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Bibliographic Details
Published in:The Journal of biological chemistry 1970-03, Vol.245 (5), p.1108-1114
Main Authors: Voll, M J, Leive, L
Format: Article
Language:English
Subjects:
Carbon Isotopes
Cell Membrane Permeability - drug effects
Centrifugation, Density Gradient
Culture Media
Dactinomycin - metabolism
Depression, Chemical
Drug Resistance, Microbial
Edetic Acid - pharmacology
Escherichia coli - drug effects
Escherichia coli - isolation & purification
Escherichia coli - metabolism
Lipopolysaccharides - metabolism
Mutation
Polysaccharides, Bacterial - metabolism
Stimulation, Chemical
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Summary:Mutants of Escherichia coli that fail to show the characteristic increase in permeability induced by brief treatment with ethylenediaminetetraacetate have been isolated. They were examined to see whether they release lipopolysaccharide as expected during such a treatment. Mutants derived from two strains released only 20 to 40% less lipopolysaccharide than their parents, but further analysis of one mutant and its parent showed that this represented reduction of a specific lipopolysaccharide fraction. Both organisms released two lipopolysaccharide components differing in sedimentation rate in sucrose density gradients, but while both released similar amounts of the faster moving component, the mutant released only about one-third as much of the slower moving component. These results suggest that release of the slower component may be required for the permeability increase induced by EDTA.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)63295-8