Quantitative Chromatographic Analysis of Methallibure in Animal Feed Mixtures

Methallibure [1-methyl-6-(1-methylallyl)-2,5-dithiobiurea] was separated from the constituents of feed extracts by partition column chromatography. The separation utilized polydextran gel as a stationary phase and a composite organic solvent as a mobile phase. The method requires no special treatmen...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1970-06, Vol.59 (6), p.835-838
Main Authors: Krol, G.J., Carney, J.F., Kho, B.T.
Format: Article
Language:English
Subjects:
Animal Feed - analysis
Animal feed mixtures-methallibure determination
Biureas - analysis
Chromatography
Column chromatography-separation
Dithizone-metal-ion interference elimination
Methallibure-determination
Methods
Solvents
Spectrophotometry
Ultraviolet Rays
UV spectrophotometry-analysis
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Summary:Methallibure [1-methyl-6-(1-methylallyl)-2,5-dithiobiurea] was separated from the constituents of feed extracts by partition column chromatography. The separation utilized polydextran gel as a stationary phase and a composite organic solvent as a mobile phase. The method requires no special treatment of the solid support and the column can be used for an indeterminate number of elutions. The observed interference of metal ions in the extraction and chromatography step was eliminated by the addition of dithizone to the extracting solvent. The procedure is quantitative and applicable to routine analysis of different commercial field samples. The chromatographic separation was tested for specificity with a thiadiazole derivative, which was prepared from methallibure, and complete separation between the two related structures was observed. The Chromatographic column, used in the analysis, yielded approximately 900 theoretical plates.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600590624