DNA-Based Identification of Spices: DNA Isolation, Whole Genome Amplification, and Polymerase Chain Reaction

Usually spices are identified morphologically using simple methods like magnifying glasses or microscopic instruments. On the other hand, molecular biological methods like the polymerase chain reaction (PCR) enable an accurate and specific detection also in complex matrices. Generally, the origins o...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2011-01, Vol.59 (2), p.513-520
Main Authors: Focke, Felix, Haase, Ilka, Fischer, Markus
Format: Article
Language:English
Subjects:
Analytical Methods
Aroma and flavouring agent industries
Base Sequence
Biological and medical sciences
DNA Primers - genetics
DNA, Plant - genetics
DNA, Plant - isolation & purification
Fish and seafood industries
Food industries
Fundamental and applied biological sciences. Psychology
Genome, Plant
Molecular Sequence Data
Phylogeny
Plants - classification
Plants - genetics
Polymerase Chain Reaction - methods
Spices - analysis
Spices - classification
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Summary:Usually spices are identified morphologically using simple methods like magnifying glasses or microscopic instruments. On the other hand, molecular biological methods like the polymerase chain reaction (PCR) enable an accurate and specific detection also in complex matrices. Generally, the origins of spices are plants with diverse genetic backgrounds and relationships. The processing methods used for the production of spices are complex and individual. Consequently, the development of a reliable DNA-based method for spice analysis is a challenging intention. However, once established, this method will be easily adapted to less difficult food matrices. In the current study, several alternative methods for the isolation of DNA from spices have been developed and evaluated in detail with regard to (i) its purity (photometric), (ii) yield (fluorimetric methods), and (iii) its amplifiability (PCR). Whole genome amplification methods were used to preamplify isolates to improve the ratio between amplifiable DNA and inhibiting substances. Specific primer sets were designed, and the PCR conditions were optimized to detect 18 spices selectively. Assays of self-made spice mixtures were performed to proof the applicability of the developed methods.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf103702s