Isolation of a Protein responsible for Aggregation of Avian Embryonic Cells

CELL adhesion, as a mechanism of morphogenesis in higher organisms, has been explained by two contrasting hypotheses: first, by adhesive macromolecules 1 , and, second, by a lyophobic colloid mechanism 2 . Lilien and Moscona 3 have reaggregated retina cells with a supernatant preparation taken from...

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Bibliographic Details
Published in:Nature (London) 1970-06, Vol.226 (5249), p.970-971
Main Authors: DADAY, H, CREASER, E. H
Format: Article
Language:English
Subjects:
Animals
Cell Adhesion
Cell Aggregation
Chick Embryo
Culture Media
Edetic Acid - pharmacology
Eye Proteins - isolation & purification
Humanities and Social Sciences
In Vitro Techniques
Isotonic Solutions
letter
multidisciplinary
Retina - cytology
Retina - embryology
Science
Science (multidisciplinary)
Trypsin
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Summary:CELL adhesion, as a mechanism of morphogenesis in higher organisms, has been explained by two contrasting hypotheses: first, by adhesive macromolecules 1 , and, second, by a lyophobic colloid mechanism 2 . Lilien and Moscona 3 have reaggregated retina cells with a supernatant preparation taken from a culture of retina cells without, however, investigating the nature of the active compound. Electron microscopy investigations 2 have suggested that there are gaps of 100–200 Å between the plasmalemmas of living chicken embryonic cells and of many tissues of a variety of species, but were unable to find any intercellular material. We have examined the reaggregation of avian cells to identify the nature of the molecules causing reaggregation if such were found to exist.
ISSN:0028-0836
1476-4687
DOI:10.1038/226970a0