Competence Mutants: Isolation of Transformation Deficient Strains of Haemophilus influenzae

BACTERIAL transformation involves several steps, beginning with the association between free DNA and the cells, and ending with alteration of the recipient genome. Although the general processes of “DNA uptake” and “integration” must be involved, the transient nature of the specific events has made...

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Bibliographic Details
Published in:Nature (London) 1970-08, Vol.227 (5257), p.515-517
Main Authors: CASTER, JOHN H, POSTEL, EDITH HORN, GOODGAL, SOL H
Format: Article
Language:English
Subjects:
Binding Sites
DNA, Bacterial - metabolism
Drug Resistance, Microbial
Haemophilus influenzae - isolation & purification
Haemophilus influenzae - metabolism
Humanities and Social Sciences
letter
multidisciplinary
Mutation
Nucleic Acid Denaturation
Science
Science (multidisciplinary)
Streptomycin
Transformation, Genetic
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Summary:BACTERIAL transformation involves several steps, beginning with the association between free DNA and the cells, and ending with alteration of the recipient genome. Although the general processes of “DNA uptake” and “integration” must be involved, the transient nature of the specific events has made their elucidation extremely difficult. Furthermore, although physiological changes occur during the development of competence, it is not known which changes are essential to transformation. We believe these processes could most readily be analysed by genetic techniques.
ISSN:0028-0836
1476-4687
DOI:10.1038/227515a0