Structure of RNA in Ribosomes

The 50S and 30S ribosomes and 23S and 16S RNA were hydrolyzed with ribonuclease A. The rate constants and number of fragments produced were determined for each reaction. The conformation of 23S RNA changes when the RNA is extracted from the ribosome. Specific regions of the RNA in 50S and 30S riboso...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1970-10, Vol.170 (3954), p.171-173
Main Authors: Hartman, Karl A., Amaya, Jaime, Schachter, Eugene M.
Format: Article
Language:English
Subjects:
Bacterial Proteins - biosynthesis
Electrophoresis, Disc
Escherichia coli - cytology
Escherichia coli - metabolism
Gels
Hydrolysis
Kinetics
Molecular Weight
Phosphates
Ribonucleases
Ribosomes
Ribosomes - metabolism
RNA
RNA - analysis
RNA, Bacterial - metabolism
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Summary:The 50S and 30S ribosomes and 23S and 16S RNA were hydrolyzed with ribonuclease A. The rate constants and number of fragments produced were determined for each reaction. The conformation of 23S RNA changes when the RNA is extracted from the ribosome. Specific regions of the RNA in 50S and 30S ribosomes are protected from hydrolysis by the ribosomal proteins.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.170.3954.171