12-Oxo-Phytodienoic Acid-Glutathione Conjugate is Transported into the Vacuole in Arabidopsis

While exogenous toxic compounds such as herbicides are thought to be sequestered into vacuoles in the form of glutathione (GSH) conjugates, little is understood about natural plant products conjugated with GSH. To identify natural products conjugated with GSH in plants, metabolites in the Arabidopsi...

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Bibliographic Details
Published in:Plant and cell physiology 2011, Vol.52 (1), p.205-209
Main Authors: Ohkama-Ohtsu, Naoko, Sasaki-Sekimoto, Yuko, Oikawa, Akira, Jikumaru, Yusuke, Shinoda, Shoko, Inoue, Eri, Kamide, Yukiko, Yokoyama, Tadashi, Hirai, Masami Yokota, Shirasu, Ken, Kamiya, Yuji, Oliver, David J, Saito, Kazuki
Format: Article
Language:English
Subjects:
Arabidopsis - metabolism
Base Sequence
DNA Primers
Fatty Acids, Unsaturated - metabolism
Glutathione - metabolism
Polymerase Chain Reaction
Vacuoles - metabolism
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Summary:While exogenous toxic compounds such as herbicides are thought to be sequestered into vacuoles in the form of glutathione (GSH) conjugates, little is understood about natural plant products conjugated with GSH. To identify natural products conjugated with GSH in plants, metabolites in the Arabidopsis γ-glutamyl transpeptidase (ggt) 4 knockout mutants that are blocked in the degradation of GSH conjugates in the vacuole were compared with those in wild-type plants. Among the metabolites identified, one was confirmed to be the 12-oxo-phytodienoic acid (OPDA)-GSH conjugate, indicating that OPDA, a precursor of jasmonic acid (JA), is transported into the vacuole as a GSH conjugate.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcq181