Rapid Quantitative Analysis of Lipids Using a Colorimetric Method in a Microplate Format

A colorimetric sulfo-phospho-vanillin (SPV) method was developed for high throughput analysis of total lipids. The developed method uses a reaction mixture that is maintained in a 96-well microplate throughout the entire assay. The new assay provides the following advantages over other methods of li...

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Bibliographic Details
Published in:Lipids 2011-01, Vol.46 (1), p.95-103
Main Authors: Cheng, Yu-Shen, Zheng, Yi, VanderGheynst, Jean S
Format: Article
Language:English
Subjects:
Benzaldehydes - chemistry
Biomedical and Life Sciences
Colorimetry - methods
Life Sciences
Lipidology
Lipids
Lipids - analysis
Macro‐gravimetric
Medical Biochemistry
Medicinal Chemistry
Methods
Microalgae
Microbial Genetics and Genomics
Neurochemistry
Nutrition
Sulfo‐phospho‐vanillin
Sulfuric acid
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Summary:A colorimetric sulfo-phospho-vanillin (SPV) method was developed for high throughput analysis of total lipids. The developed method uses a reaction mixture that is maintained in a 96-well microplate throughout the entire assay. The new assay provides the following advantages over other methods of lipid measurement: (1) background absorbance can be easily corrected for each well, (2) there is less risk of handling and transferring sulfuric acid contained in reaction mixtures, (3) color develops more consistently providing more accurate measurement of absorbance, and (4) the assay can be used for quantitative measurement of lipids extracted from a wide variety of sources. Unlike other spectrophotometric approaches that use fluorescent dyes, the optimal spectra and reaction conditions for the developed assay do not vary with the sample source. The developed method was used to measure lipids in extracts from four strains of microalgae. No significant difference was found in lipid determination when lipid content was measured using the new method and compared to results obtained using a macro-gravimetric method.
ISSN:0024-4201
1558-9307
DOI:10.1007/s11745-010-3494-0