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Calcium Binding, Quantum Yield, and Emitting Molecule in Aequorin Bioluminescence
THE protein aequorin, from the jellyfish Aequorea , emits bluish light in aqueous solution on addition specifically of calcium or strontium; the necessary energy of more than 60 kcalories/mole at the peak wavelength is evidently provided by an intramolecular reaction which is accompanied by a change...
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| Published in: | Nature (London) 1970-09, Vol.227 (5265), p.1356-1357 |
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| Main Authors: | , |
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| Language: | English |
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| container_issue | 5265 |
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| container_title | Nature (London) |
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| creator | SHIMOMURA, OSAMU JOHNSON, FRANK H |
| description | THE protein aequorin, from the jellyfish
Aequorea
, emits bluish light in aqueous solution on addition specifically of calcium or strontium; the necessary energy of more than 60 kcalories/mole at the peak wavelength is evidently provided by an intramolecular reaction which is accompanied by a change in absorption spectrum and yields a blue fluorescent reaction product
1,2
. The extreme sensitivity to Ca
2+
, much more than to Sr
2+
, provides a basis for quick microdetermination of Ca
2+
in biological fluids
3
and has been used in detecting the relation of Ca
2+
to contraction of single muscle fibres
4,5
and to activity of mitochondria
6
. The chief properties of aequorin, including the molecular weight (m.w.) of 30,000 by sedimentation, diffusion or 32,000 by amino-acid composition, have been reported
7
. The kinetics of the light-emitting reaction have also been studied
8
. |
| doi_str_mv | 10.1038/2271356a0 |
| format | article |
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Aequorea
, emits bluish light in aqueous solution on addition specifically of calcium or strontium; the necessary energy of more than 60 kcalories/mole at the peak wavelength is evidently provided by an intramolecular reaction which is accompanied by a change in absorption spectrum and yields a blue fluorescent reaction product
1,2
. The extreme sensitivity to Ca
2+
, much more than to Sr
2+
, provides a basis for quick microdetermination of Ca
2+
in biological fluids
3
and has been used in detecting the relation of Ca
2+
to contraction of single muscle fibres
4,5
and to activity of mitochondria
6
. The chief properties of aequorin, including the molecular weight (m.w.) of 30,000 by sedimentation, diffusion or 32,000 by amino-acid composition, have been reported
7
. The kinetics of the light-emitting reaction have also been studied
8
.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/2271356a0</identifier><identifier>PMID: 4393938</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Amino Acids - analysis ; Calcium ; Chromatography, Gel ; Cnidaria ; Edetic Acid ; Electrophoresis, Disc ; Fluorometry ; Humanities and Social Sciences ; letter ; Luminescent Measurements ; Molecular Weight ; multidisciplinary ; Protein Binding ; Proteins - analysis ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1970-09, Vol.227 (5265), p.1356-1357</ispartof><rights>Springer Nature Limited 1970</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-a3bcae8f063ea8e2902e7b091c7aa32f2b15e33beb40a066bcbb2d107de9aa1f3</citedby><cites>FETCH-LOGICAL-c405t-a3bcae8f063ea8e2902e7b091c7aa32f2b15e33beb40a066bcbb2d107de9aa1f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4393938$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHIMOMURA, OSAMU</creatorcontrib><creatorcontrib>JOHNSON, FRANK H</creatorcontrib><title>Calcium Binding, Quantum Yield, and Emitting Molecule in Aequorin Bioluminescence</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>THE protein aequorin, from the jellyfish
Aequorea
, emits bluish light in aqueous solution on addition specifically of calcium or strontium; the necessary energy of more than 60 kcalories/mole at the peak wavelength is evidently provided by an intramolecular reaction which is accompanied by a change in absorption spectrum and yields a blue fluorescent reaction product
1,2
. The extreme sensitivity to Ca
2+
, much more than to Sr
2+
, provides a basis for quick microdetermination of Ca
2+
in biological fluids
3
and has been used in detecting the relation of Ca
2+
to contraction of single muscle fibres
4,5
and to activity of mitochondria
6
. The chief properties of aequorin, including the molecular weight (m.w.) of 30,000 by sedimentation, diffusion or 32,000 by amino-acid composition, have been reported
7
. The kinetics of the light-emitting reaction have also been studied
8
.</description><subject>Amino Acids - analysis</subject><subject>Calcium</subject><subject>Chromatography, Gel</subject><subject>Cnidaria</subject><subject>Edetic Acid</subject><subject>Electrophoresis, Disc</subject><subject>Fluorometry</subject><subject>Humanities and Social Sciences</subject><subject>letter</subject><subject>Luminescent Measurements</subject><subject>Molecular Weight</subject><subject>multidisciplinary</subject><subject>Protein Binding</subject><subject>Proteins - analysis</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1970</creationdate><recordtype>article</recordtype><recordid>eNptkE1LAzEQhoMotVYP_gBxT4LS1Umy3Y9jW-oHVKSgB09Lkp0tKbvZmmwO_nsjLfUic5hh3oeXmZeQSwr3FHj-wFhG-SQVcESGNMnSOEnz7JgMAVgeQ87TU3Lm3AYAJjRLBmSQ8CJUPiSruWiU9m0006bSZj2OVl6YPiw-NTbVOBKmihat7vsgRq9dg8o3GGkTTfHLdzYMM901vtUGnUKj8Jyc1KJxeLHvI_LxuHifP8fLt6eX-XQZqwQmfSy4VALzGlKOIkdWAMNMQkFVJgRnNZN0gpxLlAkISFOppGQVhazCQgha8xG52flubffl0fVlq8MFTSMMdt6VeZIWlDMawNsdqGznnMW63FrdCvtdUih_4ysP8QX2am_qZYvVgdznFfS7ne6CYtZoy03nrQl__mt2vYON6L3Fg9kf8QPqAIIu</recordid><startdate>19700926</startdate><enddate>19700926</enddate><creator>SHIMOMURA, OSAMU</creator><creator>JOHNSON, FRANK H</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19700926</creationdate><title>Calcium Binding, Quantum Yield, and Emitting Molecule in Aequorin Bioluminescence</title><author>SHIMOMURA, OSAMU ; JOHNSON, FRANK H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-a3bcae8f063ea8e2902e7b091c7aa32f2b15e33beb40a066bcbb2d107de9aa1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1970</creationdate><topic>Amino Acids - analysis</topic><topic>Calcium</topic><topic>Chromatography, Gel</topic><topic>Cnidaria</topic><topic>Edetic Acid</topic><topic>Electrophoresis, Disc</topic><topic>Fluorometry</topic><topic>Humanities and Social Sciences</topic><topic>letter</topic><topic>Luminescent Measurements</topic><topic>Molecular Weight</topic><topic>multidisciplinary</topic><topic>Protein Binding</topic><topic>Proteins - analysis</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHIMOMURA, OSAMU</creatorcontrib><creatorcontrib>JOHNSON, FRANK H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHIMOMURA, OSAMU</au><au>JOHNSON, FRANK H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calcium Binding, Quantum Yield, and Emitting Molecule in Aequorin Bioluminescence</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1970-09-26</date><risdate>1970</risdate><volume>227</volume><issue>5265</issue><spage>1356</spage><epage>1357</epage><pages>1356-1357</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>THE protein aequorin, from the jellyfish
Aequorea
, emits bluish light in aqueous solution on addition specifically of calcium or strontium; the necessary energy of more than 60 kcalories/mole at the peak wavelength is evidently provided by an intramolecular reaction which is accompanied by a change in absorption spectrum and yields a blue fluorescent reaction product
1,2
. The extreme sensitivity to Ca
2+
, much more than to Sr
2+
, provides a basis for quick microdetermination of Ca
2+
in biological fluids
3
and has been used in detecting the relation of Ca
2+
to contraction of single muscle fibres
4,5
and to activity of mitochondria
6
. The chief properties of aequorin, including the molecular weight (m.w.) of 30,000 by sedimentation, diffusion or 32,000 by amino-acid composition, have been reported
7
. The kinetics of the light-emitting reaction have also been studied
8
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>4393938</pmid><doi>10.1038/2271356a0</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1970-09, Vol.227 (5265), p.1356-1357 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84691321 |
| source | Nature |
| subjects | Amino Acids - analysis Calcium Chromatography, Gel Cnidaria Edetic Acid Electrophoresis, Disc Fluorometry Humanities and Social Sciences letter Luminescent Measurements Molecular Weight multidisciplinary Protein Binding Proteins - analysis Science Science (multidisciplinary) |
| title | Calcium Binding, Quantum Yield, and Emitting Molecule in Aequorin Bioluminescence |
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