Tyrosine Transaminase Induction by Dexamethasone in a New Rat Liver Cell Line

A cell line derived from normal, adult rat liver has been established; the cells are similar to hepatocytes, as shown by electron microscopy. The addition of dexamethasone to the culture medium induced a three- to sixfold increase in the specific activity of tyrosine α-ketoglutarate transaminase; th...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1970-11, Vol.170 (3960), p.859-861
Main Authors: Gerschenson, L. E., Andersson, Myrna, Molson, Jill, Okigaki, Tohru
Format: Article
Language:English
Subjects:
Animals
Cell culture techniques
Cell growth
Cell Line - enzymology
Cell lines
Contact Inhibition
Culture Techniques
Cultured cells
Cycloheximide - pharmacology
Dactinomycin - pharmacology
Dexamethasone - pharmacology
Enzyme Induction - drug effects
Enzymes
Epithelial cells
Flasks
Hormones
Liver
Liver - enzymology
Liver cells
Microscopy, Electron
Rats
Tyrosine Transaminase - biosynthesis
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Summary:A cell line derived from normal, adult rat liver has been established; the cells are similar to hepatocytes, as shown by electron microscopy. The addition of dexamethasone to the culture medium induced a three- to sixfold increase in the specific activity of tyrosine α-ketoglutarate transaminase; this increase was inhibited by the simultaneous addition of cycloheximide or actinomycin D. The latter, when added to cells given prior treatment with dexamethasone, further enhanced the transaminase activity. Contact-inhibited cells showed a lower response to dexamethasone than exponentially growing cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.170.3960.859