Two Forms of the Nuclear Matrix-Bound p53 Protein in HEK293 Cells

:  Like many other transcription factors, the tumor suppressor protein p53 is bound to the nuclear matrix (NM). To study the interaction of p53 with the NM in more detail, we used alkaline and acidic extractions of NM proteins. It was found that there are two forms of p53, alkali‐ and acid‐soluble,...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 2006-12, Vol.1090 (1), p.177-181
Main Authors: LAPSHINA, MARIA A., PARKHOMENKO, IGOR I., TERENTIEV, ALEXEI A.
Format: Article
Language:English
Subjects:
Cell Line
Humans
nuclear matrix
Nuclear Matrix - metabolism
p53
Protein Binding
Tumor Suppressor Protein p53 - metabolism
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Summary::  Like many other transcription factors, the tumor suppressor protein p53 is bound to the nuclear matrix (NM). To study the interaction of p53 with the NM in more detail, we used alkaline and acidic extractions of NM proteins. It was found that there are two forms of p53, alkali‐ and acid‐soluble, in NM of HEK293 cells and only one alkali‐soluble form in NM of actinomycin D–treated MCF‐7 cells. We suggest that distinct forms of p53 differ either in interactions with NM proteins or in their charges.
ISSN:0077-8923
1749-6632
1930-6547
DOI:10.1196/annals.1378.019