Reprint of: Screening for hydrocarbon biodegraders in a metagenomic clone library derived from Brazilian petroleum reservoirs

Metagenomics has been successfully applied to isolate novel biocatalysts from diverse environments and functional screening has been used as the primary technique to identify clones carrying the desired traits. This work aimed at the construction and screening of a metagenomic library as a source of...

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Published in:Organic geochemistry 2010-09, Vol.41 (9), p.1067-1073
Main Authors: Vasconcellos, Suzan Pantaroto de, Angolini, Célio Fernando Figueiredo, García, Isabel Natalia Sierra, Martins Dellagnezze, Bruna, Silva, Cynthia Canedo da, Marsaioli, Anita Jocelyne, Neto, Eugenio Vaz dos Santos, de Oliveira, Valéria Maia
Format: Article
Language:English
Subjects:
Earth sciences
Earth, ocean, space
Exact sciences and technology
Geochemistry
Hydrocarbons
Sedimentary rocks
Soil and rock geochemistry
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Summary:Metagenomics has been successfully applied to isolate novel biocatalysts from diverse environments and functional screening has been used as the primary technique to identify clones carrying the desired traits. This work aimed at the construction and screening of a metagenomic library as a source of potential hydrocarbon biodegraders. A library of 31000 clones was obtained from aerobic and anaerobic enrichments generated using a biodegraded petroleum sample collected at the Potiguar Basin in Brazil as an inoculum. A total of 5000 clones were randomly selected for high throughput screening of hexadecane biodegradation, revealing 72 positive hits. These were further evaluated using gas chromatography–mass spectrometry (GC–MS), outstanding hexadecane biodegradation ability (>70%) being detected in five clones.
ISSN:0146-6380
1873-5290
DOI:10.1016/j.orggeochem.2010.08.003