Effect of Calcium Ions on Synthesis of T5-Specific Proteins

The effect of concentration of calcium ions on the capacity for phage development and on the synthesis of phage-specific proteins by complexes of T5-infected Escherichia coli F has been studied. The omission of calcium ions from a chemically defined medium results in the formation of unstable phage-...

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Bibliographic Details
Published in:The Journal of biological chemistry 1970-11, Vol.245 (22), p.5897-5903
Main Authors: Moyer, R W, Buchanan, J M
Format: Article
Language:English
Subjects:
Acrylates
Autoradiography
Calcium - pharmacology
Carbon Isotopes
Coliphages - metabolism
Electrophoresis
Escherichia coli - growth & development
Escherichia coli - metabolism
Escherichia coli - radiation effects
Gels
Radiation Effects
Viral Proteins - biosynthesis
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Summary:The effect of concentration of calcium ions on the capacity for phage development and on the synthesis of phage-specific proteins by complexes of T5-infected Escherichia coli F has been studied. The omission of calcium ions from a chemically defined medium results in the formation of unstable phage-cell complexes. At 10 -4 m calcium chloride stable complexes are formed that have the potential for phage development but are restricted in this respect until the calcium ion concentration is elevated to 10 -3 m . Class I phage proteins, which are formed for the first 6 min after protein synthesis is first allowed following infection under standard conditions, are synthesized normally by phage-cell complexes at 10 -4 m Ca ++ . However, there is no evidence for the synthesis of Class II or Class III proteins, which first appear at 5 min and 14 min, respectively. Complexes that have been arrested at this first step may reinitiate protein synthesis and viral development upon elevation of the calcium ion concentration to 10 -3 m .
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)62641-9