IgG Synthesis in Hybrid Cells from an Antibody-producing Mouse Myeloma and an L Cell Substrain

PROLIFERATING hybrid cell strains have been produced using Sendai virus to mediate fusion of a cloned, culture-adapted mouse plasmacytoma, MOPC 315 (my unpublished work with M. Potter), with the thymidine kinase deficient cell strain LM(TK − ) C1 1D (ref. 1), referred to as C1 1D. Because the hybrid...

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Bibliographic Details
Published in:Nature (London) 1970-12, Vol.228 (5276), p.1086-1087
Main Author: PERIMAN, PHILLIP
Format: Article
Language:English
Subjects:
Animals
Antibody Formation
Culture Techniques
Humanities and Social Sciences
Hybridization, Genetic
Immunoelectrophoresis
Immunoglobulin G - biosynthesis
L Cells (Cell Line) - metabolism
letter
Mice
multidisciplinary
Multiple Myeloma - metabolism
Neoplasm Proteins - metabolism
Radioimmunoassay
Science
Science (multidisciplinary)
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Summary:PROLIFERATING hybrid cell strains have been produced using Sendai virus to mediate fusion of a cloned, culture-adapted mouse plasmacytoma, MOPC 315 (my unpublished work with M. Potter), with the thymidine kinase deficient cell strain LM(TK − ) C1 1D (ref. 1), referred to as C1 1D. Because the hybrid cell strains contained soluble enzymes with the electrophoretic mobility characteristic of the inbred strains of mice from which MOPC 315 and C1 1D were derived, at least part of both parental genomes was expressed (my unpublished work with H. G. Coon). Here I report an investigation of the synthesis of immunoglobulin by clones of these hybrid cell strains.
ISSN:0028-0836
1476-4687
DOI:10.1038/2281086a0