Effect of desacyl ghrelin, obestatin and related peptides on triglyceride storage, metabolism and GHSR signaling in 3T3-L1 adipocytes

Acyl‐ghrelin (AG), desacyl‐ghrelin (DAG) and obestatin are all derived from the same gene transcript; however their plasma levels do not necessarily change in parallel. The influence of these peptides towards the development of obesity and their direct effects on adipocyte physiology has not been th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry 2011-02, Vol.112 (2), p.704-714
Main Authors: Miegueu, Pierre, St Pierre, David, Broglio, Fabio, Cianflone, Katherine
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adipocytes - cytology
Adipocytes - drug effects
Adipocytes - metabolism
Adult
Animals
Biological Transport - drug effects
Cell Proliferation - drug effects
desacyl ghrelin
ghrelin
Ghrelin - pharmacology
GHSR receptor
Glucose - metabolism
Humans
Male
Mice
Middle Aged
obestatin
Peptides - pharmacology
Receptors, Ghrelin - metabolism
triglyceride storage
Triglycerides - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acyl‐ghrelin (AG), desacyl‐ghrelin (DAG) and obestatin are all derived from the same gene transcript; however their plasma levels do not necessarily change in parallel. The influence of these peptides towards the development of obesity and their direct effects on adipocyte physiology has not been thoroughly investigated. This study was designed to evaluate the direct effects of peptides of the ghrelin family on preadipocyte proliferation, differentiation and adipocyte lipid and glucose metabolism in 3T3‐L1 cells. 3T3 cells were treated with physiological peptide concentrations for 1 h to 9 days, and the relevant assays measured. In preadipocytes, AG, GHRP‐6 and DAG stimulated proliferation, measured as 3H‐thymidine incorporation (up to 200%, P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.22983