Respiration in Isolated Fat Cells and the Effects of Epinephrine

1. A technique was devised for ready sequential sampling of lipocyte suspensions for measurement of metabolic activities. 2. With the use of this technique in paired incubations, an effect of oligomycin to decrease the Q o o 2 of fat cells to 45% of control was shown. 3. An analogy between the effec...

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Bibliographic Details
Published in:The Journal of biological chemistry 1968-05, Vol.243 (9), p.2321-2327
Main Authors: Hepp, D, Challoner, D R, Williams, R H
Format: Article
Language:English
Subjects:
Adipose Tissue - metabolism
Animals
Carbon Isotopes
Chromatography, Thin Layer
Epinephrine - pharmacology
Glucose - metabolism
In Vitro Techniques
Insulin - pharmacology
Kinetics
Lipid Metabolism
Methods
Nucleotides - biosynthesis
Oligomycins - pharmacology
Oxidative Phosphorylation
Oxygen Consumption - drug effects
Phosphates - metabolism
Phosphorus Isotopes
Rats
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Summary:1. A technique was devised for ready sequential sampling of lipocyte suspensions for measurement of metabolic activities. 2. With the use of this technique in paired incubations, an effect of oligomycin to decrease the Q o o 2 of fat cells to 45% of control was shown. 3. An analogy between the effect of oligomycin on intact cells and that described for isolated mitochondria was suggested, since the antibiotic inhibited incorporation of 32 P i into acid-soluble mononucleotides and decreased 14 CO 2 production from glucose-1- 14 C and glucose-6- 14 C. 4. Stimulation of Q o o 2 by epinephrine was shown in the presence and in the absence of oligomycin; there was no accompanying increase in 14 CO 2 production from glucose when oligomycin was present, indicating that glucose was not the substrate for the observed increase in oxygen consumption. 5. It is concluded that oligomycin inhibits oxidative phosphorylation in the fat cell. Under these conditions, ( a ) a significant portion of the respiration of the lipocyte is not linked in an obligatory fashion to phosphorylation, and ( b ) it may be inferred that the calorigenic effect of epinephrine in adipose tissue involves electron flow to oxygen not linked to phosphorylation.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)93478-2