Measurement of B-type natriuretic peptide by two assays utilizing antibodies with different epitope specificity

To compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays. Plasma samples (n = 94) from HF patients were analyzed by the novel-type “Single Epitope Sandwich” (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibra...

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Bibliographic Details
Published in:Clinical biochemistry 2011-02, Vol.44 (2), p.257-259
Main Authors: Tamm, Natalia N., Semenov, Alexander G., Seferian, Karina R., Bereznikova, Anastasia V., Murakami, MaryAnn M., Apple, Fred S., Koshkina, Ekaterina V., Krasnoselsky, Mihail I., Katrukha, Alexey G.
Format: Article
Language:English
Subjects:
Antibodies
BNP
Epitopes - immunology
Escherichia coli
Heart failure
Humans
Natriuretic Peptide, Brain - blood
Peptide Fragments - blood
Proteolytic degradation
Sandwich type immunoassays
Sensitivity and Specificity
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Summary:To compare plasma BNP values determined by a conventional-type and a novel-type of BNP assays. Plasma samples (n = 94) from HF patients were analyzed by the novel-type “Single Epitope Sandwich” (SES assay prototype) and the conventional-type Siemens ADVIA Centaur BNP assays. Both assays were calibrated using recombinant proBNP (expressed in E. coli). The SES assay measured 1.2- to 7.2-fold (2.1 ± 0.9; mean, SD) greater BNP concentrations compared to the Siemens assay. A subset of six samples (6.4%) demonstrated the largest (3- to 7.2-fold higher) difference. The SES prototype assay appears to more accurately measure the absolute concentrations of BNP immunoreactive forms.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2010.09.030