Molecular analysis of ARF1 expression profiles during development of physic nut (Jatropha curcas L.)

A cDNA clone designated arf1 was isolated from a physic nut (Jatropha curcas L.) endosperm cDNA library which encodes a small GTP-binding protein and has significant homology to ADP-ribosylation factors (ARF) in plants, animals and microbes. The cDNA contains an open reading frame that encodes a pol...

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Bibliographic Details
Published in:Molecular biology reports 2011-03, Vol.38 (3), p.1681-1686
Main Authors: Qin, Xiaobo, Lin, Fanrong, Lii, Yifan, Gou, Chunbao, Chen, Fang
Format: Article
Language:English
Subjects:
ADP-ribosylation factor
ADP-Ribosylation Factors - genetics
ADP-Ribosylation Factors - metabolism
Animal Anatomy
Animal Biochemistry
Binding sites
Biomedical and Life Sciences
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA, Complementary - genetics
Electrophoresis, Polyacrylamide Gel
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
GTP-binding protein
Guanosine Triphosphate - metabolism
Histology
Jatropha - genetics
Jatropha - growth & development
Life Sciences
Molecular biology
Molecular Sequence Data
Morphology
Nuts
Organ Specificity - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Polymerase chain reaction
Protein Binding
Proteins
Recombinant Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
RT-PCR
Sequence Analysis, Protein
Stress, Physiological - genetics
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Summary:A cDNA clone designated arf1 was isolated from a physic nut (Jatropha curcas L.) endosperm cDNA library which encodes a small GTP-binding protein and has significant homology to ADP-ribosylation factors (ARF) in plants, animals and microbes. The cDNA contains an open reading frame that encodes a polypeptide of 181 amino acids with a calculated molecular mass of 20.7 kDa. The deduced amino acid sequence showed high homology to known ARFs from other organisms. The products of the arf1 obtained by overexpression in E. coli revealed the specific binding activity toward GTP. The expression of arf1 was observed in flowers, roots, stems and leaves as analyzed by RT-PCR, and its transcriptional level was highest in flowers. In particular, the accumulation of arf1 transcripts was different under various environmental stresses in seedlings. The results suggest that arf1 plays distinct physiological roles in Jatropha curcas cells.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-010-0280-2