The Transformed Cell in Thymus-PHA Cultures: Blood or Tissue Origin?

The present study demonstrated that of the 18 million thymus, lymph node, or spleen cells in cultures containing PHA, on the average only 540, 990, and 6,170 small lymphocytes, respectively, were located in the blood vessels of these organs when the cell suspensions were prepared. Even if all of the...

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Bibliographic Details
Published in:Blood 1968-08, Vol.32 (2), p.225-230
Main Author: Schwarz, M. Roy
Format: Article
Language:English
Subjects:
Animals
Blood Vessels
Culture Techniques
DNA - analysis
Lectins
Leukocyte Count
Lymph Nodes - cytology
Lymphocytes - cytology
Lymphoid Tissue - blood supply
Mitosis
Rats
Spleen - cytology
Thymus Gland - cytology
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Summary:The present study demonstrated that of the 18 million thymus, lymph node, or spleen cells in cultures containing PHA, on the average only 540, 990, and 6,170 small lymphocytes, respectively, were located in the blood vessels of these organs when the cell suspensions were prepared. Even if all of these cells transformed in response to PHA, it is unlikely that they could account for the transformation observed. Therefore, it was concluded that the lymphocytes of the parenchyma possessed the ability to transform in response to mitogenic agents.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V32.2.225.225