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Adult brain nitrergic activity after concomitant prenatal exposure to ethanol and methyl mercury
Pregnant rats were exposed to ethanol (EtOH) and/or methyl mercury (MeHg) during fetal brain development. Nitrergic activity was quantified by densitometric measurement of formazan deposits in the hippocampus, cerebellum and striatum of two-month-old offspring following histochemical assay for NADPH...
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| Published in: | Acta histochemica 2010-11, Vol.112 (6), p.583-591 |
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| creator | Maia, Cristiane do Socorro Ferraz Ferreira, Vânia Maria Moraes Kahwage, Rafael Lima do Amaral, Michelle Nunes Serra, Raphael Borges Noro dos Santos, Setsuko do Nascimento, José Luiz Martins Rodrigues, Lorena Gonçalves Trévia, Nonata Diniz, Cristovam Wanderley Picanço |
| description | Pregnant rats were exposed to ethanol (EtOH) and/or methyl mercury (MeHg) during fetal brain development. Nitrergic activity was quantified by densitometric measurement of formazan deposits in the hippocampus, cerebellum and striatum of two-month-old offspring following histochemical assay for NADPH-diaphorase (NADPH-d) activity. Compared to control subjects, an increase in nitrergic activity was found in the molecular layer of dentate gyrus and in the lacunosum molecular and stratum radiatum of CA1 (
cornus amoni 1) in the EtOH+MeHg group, whereas a single administration of EtOH increased the activity in all striatal segments. The cerebellum seems to be less sensitive at this time-point to intoxication, and presented an increase only at the molecular layer of EtOH-exposed animals when compared to the MeHg and EtOH+MeHg groups (ANOVA, one-way followed by Tukey's test,
p |
| doi_str_mv | 10.1016/j.acthis.2009.06.004 |
| format | article |
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cornus amoni 1) in the EtOH+MeHg group, whereas a single administration of EtOH increased the activity in all striatal segments. The cerebellum seems to be less sensitive at this time-point to intoxication, and presented an increase only at the molecular layer of EtOH-exposed animals when compared to the MeHg and EtOH+MeHg groups (ANOVA, one-way followed by Tukey's test,
p<0.05 or
p<0.01). Taken together, results suggest that developmental exposure to EtOH and MeHg, singularly or in combination, alters nitrergic activity in adult rat in different ways depending on the region and layer of the central nervous system (CNS), and that these alterations might be related to different local metabolic properties.</description><identifier>ISSN: 0065-1281</identifier><identifier>EISSN: 1618-0372</identifier><identifier>DOI: 10.1016/j.acthis.2009.06.004</identifier><identifier>PMID: 19748654</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Adults ; Analysis of variance ; Animals ; Brain ; Brain - drug effects ; Brain - metabolism ; Brain development ; Cerebellum ; Cornus ; Densitometers ; Ethanol ; Ethanol - administration & dosage ; Ethanol - toxicity ; Ethyl alcohol ; Female ; Immunohistochemistry ; Male ; Mercury ; Methyl mercury ; Methylmercury Compounds - administration & dosage ; Methylmercury Compounds - toxicity ; NADPH Dehydrogenase - metabolism ; Nitrergic activity ; Nitric Oxide - metabolism ; Pregnancy ; Prenatal Exposure Delayed Effects ; Rat ; Rats ; Rats, Wistar</subject><ispartof>Acta histochemica, 2010-11, Vol.112 (6), p.583-591</ispartof><rights>2009 Elsevier GmbH</rights><rights>Copyright © 2009 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-b7eb79c3f452258720fad2c8249f3d1ff619c37e05cadd30e36ead4170be3cd23</citedby><cites>FETCH-LOGICAL-c426t-b7eb79c3f452258720fad2c8249f3d1ff619c37e05cadd30e36ead4170be3cd23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19748654$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maia, Cristiane do Socorro Ferraz</creatorcontrib><creatorcontrib>Ferreira, Vânia Maria Moraes</creatorcontrib><creatorcontrib>Kahwage, Rafael Lima</creatorcontrib><creatorcontrib>do Amaral, Michelle Nunes</creatorcontrib><creatorcontrib>Serra, Raphael Borges</creatorcontrib><creatorcontrib>Noro dos Santos, Setsuko</creatorcontrib><creatorcontrib>do Nascimento, José Luiz Martins</creatorcontrib><creatorcontrib>Rodrigues, Lorena Gonçalves</creatorcontrib><creatorcontrib>Trévia, Nonata</creatorcontrib><creatorcontrib>Diniz, Cristovam Wanderley Picanço</creatorcontrib><title>Adult brain nitrergic activity after concomitant prenatal exposure to ethanol and methyl mercury</title><title>Acta histochemica</title><addtitle>Acta Histochem</addtitle><description>Pregnant rats were exposed to ethanol (EtOH) and/or methyl mercury (MeHg) during fetal brain development. Nitrergic activity was quantified by densitometric measurement of formazan deposits in the hippocampus, cerebellum and striatum of two-month-old offspring following histochemical assay for NADPH-diaphorase (NADPH-d) activity. Compared to control subjects, an increase in nitrergic activity was found in the molecular layer of dentate gyrus and in the lacunosum molecular and stratum radiatum of CA1 (
cornus amoni 1) in the EtOH+MeHg group, whereas a single administration of EtOH increased the activity in all striatal segments. The cerebellum seems to be less sensitive at this time-point to intoxication, and presented an increase only at the molecular layer of EtOH-exposed animals when compared to the MeHg and EtOH+MeHg groups (ANOVA, one-way followed by Tukey's test,
p<0.05 or
p<0.01). Taken together, results suggest that developmental exposure to EtOH and MeHg, singularly or in combination, alters nitrergic activity in adult rat in different ways depending on the region and layer of the central nervous system (CNS), and that these alterations might be related to different local metabolic properties.</description><subject>Adults</subject><subject>Analysis of variance</subject><subject>Animals</subject><subject>Brain</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Brain development</subject><subject>Cerebellum</subject><subject>Cornus</subject><subject>Densitometers</subject><subject>Ethanol</subject><subject>Ethanol - administration & dosage</subject><subject>Ethanol - toxicity</subject><subject>Ethyl alcohol</subject><subject>Female</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Mercury</subject><subject>Methyl mercury</subject><subject>Methylmercury Compounds - administration & dosage</subject><subject>Methylmercury Compounds - toxicity</subject><subject>NADPH Dehydrogenase - metabolism</subject><subject>Nitrergic activity</subject><subject>Nitric Oxide - metabolism</subject><subject>Pregnancy</subject><subject>Prenatal Exposure Delayed Effects</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhi0EosvCP0DIN3pJGH_ETi5IVUUpUiUu9Ow69oR6lY_Fdir23-PVrsStPY0sP_POaB5CPjKoGTD1ZVdblx9DqjlAV4OqAeQrsmGKtRUIzV-TDYBqKsZbdkHepbSDAoLgb8kF67RsVSM35OHKr2OmfbRhpnPIEePv4GiJDk8hH6gdMkbqltktU8h2znQfcbbZjhT_7pe0RqR5oZgf7byM1M6eTuVxGEuJbo2H9-TNYMeEH851S-5vvv26vq3ufn7_cX11VznJVa56jb3unBhkw3nTag6D9dy1XHaD8GwYFCu_GqFx1nsBKBRaL5mGHoXzXGzJ51PuPi5_VkzZTCE5HEc747Im08pONkp0zYukLhi0x1NtyeWzJFOalW11cwyVJ9TFJaWIg9nHMNl4MAzM0ZfZmZMvc_RlQJniq7R9Ok9Y-wn9_6azoAJ8PQFYbvcUMJrkAs4OfYjosvFLeH7CP60WqcM</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>Maia, Cristiane do Socorro Ferraz</creator><creator>Ferreira, Vânia Maria Moraes</creator><creator>Kahwage, Rafael Lima</creator><creator>do Amaral, Michelle Nunes</creator><creator>Serra, Raphael Borges</creator><creator>Noro dos Santos, Setsuko</creator><creator>do Nascimento, José Luiz Martins</creator><creator>Rodrigues, Lorena Gonçalves</creator><creator>Trévia, Nonata</creator><creator>Diniz, Cristovam Wanderley Picanço</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>201011</creationdate><title>Adult brain nitrergic activity after concomitant prenatal exposure to ethanol and methyl mercury</title><author>Maia, Cristiane do Socorro Ferraz ; Ferreira, Vânia Maria Moraes ; Kahwage, Rafael Lima ; do Amaral, Michelle Nunes ; Serra, Raphael Borges ; Noro dos Santos, Setsuko ; do Nascimento, José Luiz Martins ; Rodrigues, Lorena Gonçalves ; Trévia, Nonata ; Diniz, Cristovam Wanderley Picanço</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-b7eb79c3f452258720fad2c8249f3d1ff619c37e05cadd30e36ead4170be3cd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adults</topic><topic>Analysis of variance</topic><topic>Animals</topic><topic>Brain</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>Brain development</topic><topic>Cerebellum</topic><topic>Cornus</topic><topic>Densitometers</topic><topic>Ethanol</topic><topic>Ethanol - administration & dosage</topic><topic>Ethanol - toxicity</topic><topic>Ethyl alcohol</topic><topic>Female</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Mercury</topic><topic>Methyl mercury</topic><topic>Methylmercury Compounds - administration & dosage</topic><topic>Methylmercury Compounds - toxicity</topic><topic>NADPH Dehydrogenase - metabolism</topic><topic>Nitrergic activity</topic><topic>Nitric Oxide - metabolism</topic><topic>Pregnancy</topic><topic>Prenatal Exposure Delayed Effects</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maia, Cristiane do Socorro Ferraz</creatorcontrib><creatorcontrib>Ferreira, Vânia Maria Moraes</creatorcontrib><creatorcontrib>Kahwage, Rafael Lima</creatorcontrib><creatorcontrib>do Amaral, Michelle Nunes</creatorcontrib><creatorcontrib>Serra, Raphael Borges</creatorcontrib><creatorcontrib>Noro dos Santos, Setsuko</creatorcontrib><creatorcontrib>do Nascimento, José Luiz Martins</creatorcontrib><creatorcontrib>Rodrigues, Lorena Gonçalves</creatorcontrib><creatorcontrib>Trévia, Nonata</creatorcontrib><creatorcontrib>Diniz, Cristovam Wanderley Picanço</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maia, Cristiane do Socorro Ferraz</au><au>Ferreira, Vânia Maria Moraes</au><au>Kahwage, Rafael Lima</au><au>do Amaral, Michelle Nunes</au><au>Serra, Raphael Borges</au><au>Noro dos Santos, Setsuko</au><au>do Nascimento, José Luiz Martins</au><au>Rodrigues, Lorena Gonçalves</au><au>Trévia, Nonata</au><au>Diniz, Cristovam Wanderley Picanço</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adult brain nitrergic activity after concomitant prenatal exposure to ethanol and methyl mercury</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>2010-11</date><risdate>2010</risdate><volume>112</volume><issue>6</issue><spage>583</spage><epage>591</epage><pages>583-591</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>Pregnant rats were exposed to ethanol (EtOH) and/or methyl mercury (MeHg) during fetal brain development. Nitrergic activity was quantified by densitometric measurement of formazan deposits in the hippocampus, cerebellum and striatum of two-month-old offspring following histochemical assay for NADPH-diaphorase (NADPH-d) activity. Compared to control subjects, an increase in nitrergic activity was found in the molecular layer of dentate gyrus and in the lacunosum molecular and stratum radiatum of CA1 (
cornus amoni 1) in the EtOH+MeHg group, whereas a single administration of EtOH increased the activity in all striatal segments. The cerebellum seems to be less sensitive at this time-point to intoxication, and presented an increase only at the molecular layer of EtOH-exposed animals when compared to the MeHg and EtOH+MeHg groups (ANOVA, one-way followed by Tukey's test,
p<0.05 or
p<0.01). Taken together, results suggest that developmental exposure to EtOH and MeHg, singularly or in combination, alters nitrergic activity in adult rat in different ways depending on the region and layer of the central nervous system (CNS), and that these alterations might be related to different local metabolic properties.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>19748654</pmid><doi>10.1016/j.acthis.2009.06.004</doi><tpages>9</tpages></addata></record> |
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| ispartof | Acta histochemica, 2010-11, Vol.112 (6), p.583-591 |
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| source | ScienceDirect Freedom Collection |
| subjects | Adults Analysis of variance Animals Brain Brain - drug effects Brain - metabolism Brain development Cerebellum Cornus Densitometers Ethanol Ethanol - administration & dosage Ethanol - toxicity Ethyl alcohol Female Immunohistochemistry Male Mercury Methyl mercury Methylmercury Compounds - administration & dosage Methylmercury Compounds - toxicity NADPH Dehydrogenase - metabolism Nitrergic activity Nitric Oxide - metabolism Pregnancy Prenatal Exposure Delayed Effects Rat Rats Rats, Wistar |
| title | Adult brain nitrergic activity after concomitant prenatal exposure to ethanol and methyl mercury |
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