Clerodendron glandulosum.Coleb extract ameliorates high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis

• Published in: Food and chemical toxicology 2010-12, Vol.48 (12), p.3424-3431
• Main Authors: Jadeja, Ravirajsinh N., Thounaojam, Menaka C., Dandekar, Deven S., Devkar, Ranjitsinh V., Ramachandran, A.V.
• Format: Article
• Language: English
• Subjects: Alanine Transaminase - blood; Animals; Antioxidants - analysis; Antioxidants - pharmacology; Aspartate Aminotransferases - blood; Biological and medical sciences; Cell Line; Cell Survival - drug effects; Clerodendron glandulosum .Coleb; Clerodendrum - chemistry; Diet; Dietary Fats - antagonists & inhibitors; Dietary Fats - toxicity; Fatty Acids - antagonists & inhibitors; Fatty Acids - toxicity; Fatty Liver - chemically induced; Fatty Liver - drug therapy; Fatty Liver - pathology; Gastroenterology. Liver. Pancreas. Abdomen; HepG2 cells; Lipid Metabolism - drug effects; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - metabolism; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Mice; Mice, Inbred C57BL; Mitochondria, Liver - drug effects; Mitochondria, Liver - metabolism; Mitochondria, Liver - pathology; Non-alcoholic steatohepatitis; Oleic acid; Other diseases. Semiology; Oxidative stress; Oxidative Stress - drug effects; Plant Extracts - pharmacology; Reactive Oxygen Species - metabolism
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2010.09.015

This study evaluates the protective role of Clerodendron glandulosum.Coleb (CG) aqueous extract against high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis (NASH). Supplementation of NASH mice with CG extract (1% and 3% in high fat diet for 16 weeks) prevented high fat diet induced elevation in liver enzymes, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status and histopathological damage to hepatocytes. Furthermore, results from in vitro study indicates, addition of CG extract (20–200 μg/ml for 24 h) to HepG2 cells minimizes oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that CG extract has the potential of preventing high fat/fatty acid induced NASH.