Fiber-optic particle plasmon resonance sensor for detection of interleukin-1b in synovial fluids

A facile and label-free biosensing method has been developed for determining an osteoarthritis concerned cytokine, interleukin-1b (IL-1b), in synovial fluids. The biosensing technique, fiber-optic particle plasmon resonance (FOPPR), is based on gold nanoparticles-modified optical fiber where the gol...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2010-11, Vol.26 (3), p.1036-1042
Main Authors: Chiang, Chang-Yue, Hsieh, Ming-Lung, Huang, Kuo-Wei, Chau, Lai-Kwan, Chang, Chia-Ming, Lyu, Shaw-Ruey
Format: Article
Language:English
Online Access:Get full text
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Summary:A facile and label-free biosensing method has been developed for determining an osteoarthritis concerned cytokine, interleukin-1b (IL-1b), in synovial fluids. The biosensing technique, fiber-optic particle plasmon resonance (FOPPR), is based on gold nanoparticles-modified optical fiber where the gold nanoparticle surface has been modified by a mixed self-assembled monolayer for further conjugation of anti-IL-1b antibody and minimization of nonspecific adsorption. Upon binding of IL-1b to anti-IL-1b on the gold nanoparticle surface, the absorbance of the gold nanoparticle layer on the optical fiber changes and the signal change is enhanced through multiple total internal reflections along the optical fiber. Results show that the detection of IL-1b in synovial fluid by this sensor agrees quantitatively with the clinically accepted enzyme-linked immunosorbent assay (ELISA) method but a much shorter analysis time is required (
ISSN:0956-5663
DOI:10.1016/j.bios.2010.08.047