Determination of azinphos-methyl and parathion-methyl in honey by stripping voltammetry

• Published in: Electrochimica acta 2010-12, Vol.56 (1), p.566-574
• Main Authors: Tsiafoulis, Constantinos G., Nanos, Christos G.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Calibration; Confectionery products and chocolate industries, honey; Deposition; Electrodes; Food industries; Fundamental and applied biological sciences. Psychology; Honey; Mathematical analysis; Organophosphorus insecticides; Pesticides; Quantitation protocol; Standard addition method; Stripping; Voltammetry
• ISSN: 0013-4686; 1873-3859
• DOI: 10.1016/j.electacta.2010.09.009

An electrochemical method for the determination of azinphos-methyl and parathion-methyl in honey is presented. The determination is established by adsorptive stripping differential pulse voltammetry at hanging mercury working electrode. In contrast to the chromatographic methods for the determination of pesticide residues, the sample preparation of the proposed method is minimal; analytes were extracted from honey samples with a mixture of (acetone):(Britton–Robinson buffer) and then were analyzed without any additional pretreatment. The response of the analytes either individually or as a mixture was studied for a series of deposition time and molar ratio. Two quantitation protocols were compared, using either the external calibration or the standard addition method. Accuracy was tested with spiked honey samples obtaining good recovery values. The limit of detection for the honey sample (for deposition time of 10 s) was calculated 51.71 μg kg −1 for MeP and 65.87 μg kg −1 for AzMet.