Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical sig...

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Bibliographic Details
Published in:Lab on a chip 2010-01, Vol.10 (4), p.438-445
Main Authors: Shafiee, Hadi, Sano, Michael B, Henslee, Erin A, Caldwell, John L, Davalos, Rafael V
Format: Article
Language:English
Subjects:
Animals
Cell Death
Cell Line, Tumor
Cell Separation - instrumentation
Cell Separation - methods
Cell Survival
Devices
Dielectrophoresis
Electric Conductivity
Electric fields
Electric potential
Electrodes
Electrophoresis
Enrichment
Humans
Microfluidics
Signatures
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Summary:Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures. The effect of different voltages and frequencies on the gradient of the electric field and device performance was investigated numerically and validated experimentally. With these prototype devices we were able to achieve greater than 95% removal efficiency at 0.2-0.5 mm s(-1) with 100% selectivity between live and dead cells. In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells.
ISSN:1473-0197
1473-0189
DOI:10.1039/b920590j