Synaptosomes: Different Populations storing Catecholamines and Gamma-aminobutyric Acid in Homogenates of Rat Brain

IT is well established that when brain tissue is homogenized large numbers of nerve terminals pinch off to form intact membrane-bound particles known as “synaptosomes”. These can be isolated from brain homogenates by density gradient centrifugation 1–3 . Such particles contain various presumptive tr...

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Bibliographic Details
Published in:Nature (London) 1968-11, Vol.220 (5169), p.796-798
Main Authors: IVERSEN, LESLIE L, SNYDER, SOLOMON H
Format: Article
Language:English
Subjects:
Aminobutyrates - metabolism
Animals
Basal Ganglia - metabolism
Brain - cytology
Brain - metabolism
Carbon Isotopes
Centrifugation, Density Gradient
Dopamine - metabolism
Female
Humanities and Social Sciences
Hypothalamus - metabolism
In Vitro Techniques
letter
multidisciplinary
Nerve Endings - cytology
Norepinephrine - metabolism
Organoids - metabolism
Rats
Science
Science (multidisciplinary)
Tritium
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Summary:IT is well established that when brain tissue is homogenized large numbers of nerve terminals pinch off to form intact membrane-bound particles known as “synaptosomes”. These can be isolated from brain homogenates by density gradient centrifugation 1–3 . Such particles contain various presumptive transmitter substances such as acetylcholine, noradrenaline (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) 1–3 . Knowledge of synaptic organization could be greatly advanced if a differential morphology for nerve terminals using various transmitters could be established. Attempts to separate synaptosomes storing different transmitters, however, have met with only limited success 4–6 .
ISSN:0028-0836
1476-4687
DOI:10.1038/220796a0