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Synaptosomes: Different Populations storing Catecholamines and Gamma-aminobutyric Acid in Homogenates of Rat Brain
IT is well established that when brain tissue is homogenized large numbers of nerve terminals pinch off to form intact membrane-bound particles known as “synaptosomes”. These can be isolated from brain homogenates by density gradient centrifugation 1–3 . Such particles contain various presumptive tr...
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| Published in: | Nature (London) 1968-11, Vol.220 (5169), p.796-798 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c401t-f0d45fd3129607275ce20e4104d61b69da10233a7e999c389e8827685f716e843 |
| container_end_page | 798 |
| container_issue | 5169 |
| container_start_page | 796 |
| container_title | Nature (London) |
| container_volume | 220 |
| creator | IVERSEN, LESLIE L SNYDER, SOLOMON H |
| description | IT is well established that when brain tissue is homogenized large numbers of nerve terminals pinch off to form intact membrane-bound particles known as “synaptosomes”. These can be isolated from brain homogenates by density gradient centrifugation
1–3
. Such particles contain various presumptive transmitter substances such as acetylcholine, noradrenaline (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT)
1–3
. Knowledge of synaptic organization could be greatly advanced if a differential morphology for nerve terminals using various transmitters could be established. Attempts to separate synaptosomes storing different transmitters, however, have met with only limited success
4–6
. |
| doi_str_mv | 10.1038/220796a0 |
| format | article |
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1–3
. Such particles contain various presumptive transmitter substances such as acetylcholine, noradrenaline (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT)
1–3
. Knowledge of synaptic organization could be greatly advanced if a differential morphology for nerve terminals using various transmitters could be established. Attempts to separate synaptosomes storing different transmitters, however, have met with only limited success
4–6
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1–3
. Such particles contain various presumptive transmitter substances such as acetylcholine, noradrenaline (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT)
1–3
. Knowledge of synaptic organization could be greatly advanced if a differential morphology for nerve terminals using various transmitters could be established. Attempts to separate synaptosomes storing different transmitters, however, have met with only limited success
4–6
.</description><subject>Aminobutyrates - metabolism</subject><subject>Animals</subject><subject>Basal Ganglia - metabolism</subject><subject>Brain - cytology</subject><subject>Brain - metabolism</subject><subject>Carbon Isotopes</subject><subject>Centrifugation, Density Gradient</subject><subject>Dopamine - metabolism</subject><subject>Female</subject><subject>Humanities and Social Sciences</subject><subject>Hypothalamus - metabolism</subject><subject>In Vitro Techniques</subject><subject>letter</subject><subject>multidisciplinary</subject><subject>Nerve Endings - cytology</subject><subject>Norepinephrine - metabolism</subject><subject>Organoids - metabolism</subject><subject>Rats</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Tritium</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><recordid>eNptkE1LxDAQhoMoun6Af0DJSfRQnbRpknrT9RMExY9zybZTjbRJTdLD_nu77LonTwPzPvPCPIQcMjhnkKmLNAVZCA0bZMK4FAkXSm6SCUCqElCZ2CG7IXwDQM4k3ybbuSiUzPMJ8W9zq_voguswXNIb0zTo0Ub64vqh1dE4G2iIzhv7Sac6YvXlWt0Zi4FqW9N73XU6WSzcbIhzbyp6VZmaGksfXOc-0Y43gbqGvupIr702dp9sNboNeLCae-Tj7vZ9-pA8Pd8_Tq-ekooDi0kDNc-bOmNpIUCmMq8wBeQMeC3YTBS1ZpBmmZZYFEWVqQKVSqVQeSOZQMWzPXKy7O29-xkwxLIzocK21RbdEEqVA3Am5QieLsHKuxA8NmXvTaf9vGRQLvSWf3pH9GjVOcw6rNfgyueYny3z0C-MoS-_3eDt-OZ_XcdLdlQ0eFx3rYFfCt6MOQ</recordid><startdate>19681123</startdate><enddate>19681123</enddate><creator>IVERSEN, LESLIE L</creator><creator>SNYDER, SOLOMON H</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19681123</creationdate><title>Synaptosomes: Different Populations storing Catecholamines and Gamma-aminobutyric Acid in Homogenates of Rat Brain</title><author>IVERSEN, LESLIE L ; SNYDER, SOLOMON H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-f0d45fd3129607275ce20e4104d61b69da10233a7e999c389e8827685f716e843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Aminobutyrates - metabolism</topic><topic>Animals</topic><topic>Basal Ganglia - metabolism</topic><topic>Brain - cytology</topic><topic>Brain - metabolism</topic><topic>Carbon Isotopes</topic><topic>Centrifugation, Density Gradient</topic><topic>Dopamine - metabolism</topic><topic>Female</topic><topic>Humanities and Social Sciences</topic><topic>Hypothalamus - metabolism</topic><topic>In Vitro Techniques</topic><topic>letter</topic><topic>multidisciplinary</topic><topic>Nerve Endings - cytology</topic><topic>Norepinephrine - metabolism</topic><topic>Organoids - metabolism</topic><topic>Rats</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>IVERSEN, LESLIE L</creatorcontrib><creatorcontrib>SNYDER, SOLOMON H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>IVERSEN, LESLIE L</au><au>SNYDER, SOLOMON H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synaptosomes: Different Populations storing Catecholamines and Gamma-aminobutyric Acid in Homogenates of Rat Brain</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1968-11-23</date><risdate>1968</risdate><volume>220</volume><issue>5169</issue><spage>796</spage><epage>798</epage><pages>796-798</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>IT is well established that when brain tissue is homogenized large numbers of nerve terminals pinch off to form intact membrane-bound particles known as “synaptosomes”. These can be isolated from brain homogenates by density gradient centrifugation
1–3
. Such particles contain various presumptive transmitter substances such as acetylcholine, noradrenaline (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT)
1–3
. Knowledge of synaptic organization could be greatly advanced if a differential morphology for nerve terminals using various transmitters could be established. Attempts to separate synaptosomes storing different transmitters, however, have met with only limited success
4–6
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| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1968-11, Vol.220 (5169), p.796-798 |
| issn | 0028-0836 1476-4687 |
| language | eng |
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| source | Nature |
| subjects | Aminobutyrates - metabolism Animals Basal Ganglia - metabolism Brain - cytology Brain - metabolism Carbon Isotopes Centrifugation, Density Gradient Dopamine - metabolism Female Humanities and Social Sciences Hypothalamus - metabolism In Vitro Techniques letter multidisciplinary Nerve Endings - cytology Norepinephrine - metabolism Organoids - metabolism Rats Science Science (multidisciplinary) Tritium |
| title | Synaptosomes: Different Populations storing Catecholamines and Gamma-aminobutyric Acid in Homogenates of Rat Brain |
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