Somatic Cell Hybrid between the Established Human Line D98 (Presumptive HeLa) and 3T3

Somatic cell hybrids have been made between an established human cell line with a long culture history and an established mouse fibroblast line. When first analyzed, the hybrid cells contained nearly twice as many mouse chromosomes as the mouse parent line, and a human chromosome complement of about...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1969-02, Vol.163 (3868), p.697-698
Main Authors: Matsuya, Yutaka, Green, Howard
Format: Article
Language:English
Subjects:
Animals
Cell lines
Cells
Chromosomes
Clone Cells
Culture Techniques
Diploidy
DNA
Fibroblasts
Genes
HeLa Cells
Human chromosomes
Humans
Hybrid cells
Hybridity
Hybridization, Genetic
Karyotyping
L cells
Mice
Somatic cells
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Summary:Somatic cell hybrids have been made between an established human cell line with a long culture history and an established mouse fibroblast line. When first analyzed, the hybrid cells contained nearly twice as many mouse chromosomes as the mouse parent line, and a human chromosome complement of about half that of the human parent. There was further loss of human chromosomes on continued cultivation. This behavior resembles that of other human-mouse hybrids and appears to be characteristic of the human-mouse combination. However, the number of human chromosomes is greater than in hybrids made from human diploid fibroblasts. Some clones contain more than a haploid quantity of human DNA per cell and should synthesize a much greater number of human gene products.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.163.3868.697