Therapeutic effects of Baicalein on atopic dermatitis-like skin lesions of NC/Nga mice induced by dermatophagoides pteronyssinus

• Published in: International immunopharmacology 2010-09, Vol.10 (9), p.1142-1148
• Main Authors: Yun, Mi-Young, Yang, Jae-Heon, Kim, Dae-Keun, Cheong, Kwang-Jo, Song, Hyang-Hee, Kim, Dong-Hee, Cheong, Kyu-Jin, Kim, Young-Il, Shin, Sang-Chul
• Format: Article
• Language: English
• Subjects: AD; Allergic diseases; Animals; Anti-Inflammatory Agents - immunology; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antigens, CD - analysis; Antigens, CD - immunology; Antigens, Differentiation, T-Lymphocyte - analysis; Antigens, Differentiation, T-Lymphocyte - immunology; Atopic dermatitis; Baicalein; BE; Biological and medical sciences; CD11 Antigens - analysis; CD11 Antigens - immunology; CD3 Complex - analysis; CD3 Complex - immunology; Cells, Cultured; Dermatitis, Atopic - drug therapy; Dermatitis, Atopic - immunology; Dermatitis, Atopic - pathology; Dermatophagoides pteronyssinus; Dermatophagoides pteronyssinus - immunology; Female; Flavanones - immunology; Flavanones - pharmacology; Flavanones - therapeutic use; Immune cells; Immunoglobulin E - analysis; Immunoglobulin E - immunology; Immunopathology; Inflammation - immunology; Inflammation - pathology; Inflammation Mediators - analysis; Inflammation Mediators - immunology; Inflammatory cytokines; Interferon-gamma - immunology; Interleukin-6 - blood; Interleukin-6 - immunology; Lectins, C-Type - analysis; Lectins, C-Type - immunology; Leukocyte Common Antigens - analysis; Leukocyte Common Antigens - immunology; Medical sciences; Mice; NC/Nga mice; Pharmacology. Drug treatments; Receptors, CCR3 - analysis; Receptors, CCR3 - immunology; Severity of Illness Index; Skin - drug effects; Skin - immunology; Skin - pathology; Skin allergic diseases. Stinging insect allergies; Spleen - drug effects; Spleen - immunology; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - immunology; Up-Regulation - drug effects; Up-Regulation - immunology
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2010.06.020

The present study was conducted to investigate the effects of Baicalein (BE), which is hydrolyzed product of Baicalin (BA), on atopic dermatitis (AD). AD was induced in NC/Nga mice by DPE treatment. BE hydrogels treatment reduced the levels of skin severity scores. BE hydrogels treatment also decreased inflammatory cytokines such as TNF-α, IL-6, and its level in the serum. BE hydrogels treatment elevated IFN-γ level in the spleenocyte culture supernatant. Cell numbers in the skin positive to CD3+/CD69+, CCR3+, CD11b+/Gr-1+, B220+/IgE+ all of which were up-regulated in AD-induced mice were decreased and returned to normal levels. Histological examination showed that infiltration levels of immune cells in the skin of AD-induced NC/Nga mice were much improved by BE hydrogels treatment. These results thus suggest that BE can regulate molecular mediators and immune cells that are functionally associated with atopic dermatitis induced in NC/Nga mice, and may play an important role in recovering AD symptoms. The inhibitory effects of BE hydrogel on the expression of cytokines in atopic dermatitis imply that it could be developed as a novel anti-atopic therapy. [Display omitted] ► The values of clinical skin by BE hydrogel treatment decreased at 8 and 16 weeks. ► The immune cell isolated from NC/Nga mice skin by BE hydrogel treatment decreased significantly. ► BE hydrogel treatment decreased inflammatory cytokines such as TNF-α, IL-6, levels in the serum. ► BE can regulate molecular mediators and immune cells and recovers atopic dermatitis symptoms.