Purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures

• Published in: Bioresource technology 2011, Vol.102 (2), p.1795-1802
• Main Authors: Parmar, Asha, Singh, Niraj Kumar, Kaushal, Avani, Sonawala, Sagar, Madamwar, Datta
• Format: Article
• Language: English
• Subjects: Absorption spectra; Bacterial Proteins - metabolism; Bands; Biological and medical sciences; Culture; Cyanobacteria; Cyanobacteria - metabolism; Electrophoresis, Polyacrylamide Gel; Emission spectra; Fluorescence; Fundamental and applied biological sciences. Psychology; Halomicronema sp. A32DM; Lyngbya; Lyngbya sp. A09DM; Marine; Phormidium; Phormidium sp. A27DM; Phycoerythrin; Phycoerythrin - isolation & purification; Phycoerythrin - metabolism; Polyethylenes; Purification; Seawater - microbiology; Silver Staining; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2010.09.025

The present study is focused on purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures – Phormidium sp. A27DM, Lyngbya sp. A09DM and Halomicronema sp. A32DM. ‘Phycoerythrin’ was successfully purified and characterized. On SDS–PAGE, the PE purified from all three young cultures showed four bands – corresponding to α and β subunits of each of PE-I and PE-II. However, phycoerythrin purified after prolonged growth of Phormidium sp. A27DM and Halomicronema sp. A32DM showed only one band corresponding to 14 kDa whereas Lyngbya sp. A09DM continued to produce uncleaved phycoerythrin. The absorption spectra of purified PEs from all the three young and old cultures showed variations however the fluorescence studies of the purified PEs in all cases gave the emission spectra at around 580 nm. The described work is of great importance to understand the role of phycoerythrin in adapting cyanobacteria to stress conditions.