Identification of Blue-Light Photoresponse Genes in Oyster Mushroom Mycelia

Using the characteristics of light-emitting diodes, we studied the effects of light quality, light intensity, and repeated light-dark cycles on the growth of oyster mushroom mycelia. Blue-light stimulation suppressed growth in oyster mushroom mycelia, and this effect was remarkably dependent on ligh...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (10), p.2160-2165
Main Authors: NAKANO, Yoko, FUJII, Hiroshi, KOJIMA, Masanobu
Format: Article
Language:English
Subjects:
Biological and medical sciences
blue-light stimulation
Color
differential display analysis
Dose-Response Relationship, Radiation
Down-Regulation - radiation effects
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Fungal - genetics
Gene Expression Regulation, Fungal - radiation effects
Genes, Fungal - genetics
Light
light-emitting diodes
Mycelium - genetics
Mycelium - growth & development
Mycelium - radiation effects
oyster mushroom mycelia
photoresponse genes
Pleurotus - genetics
Pleurotus - growth & development
Pleurotus - radiation effects
Up-Regulation - radiation effects
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Summary:Using the characteristics of light-emitting diodes, we studied the effects of light quality, light intensity, and repeated light-dark cycles on the growth of oyster mushroom mycelia. Blue-light stimulation suppressed growth in oyster mushroom mycelia, and this effect was remarkably dependent on light intensity. Random amplified polymorphic DNA differential display analysis enabled the identification for the first time of 15 upregulated and 13 downregulated genes induced by blue-light stimulation. On the basis of a homologous search using BLASTX and the Interpro database, the transcription products were analyzed to identify the putative proteins. In addition, by real-time PCR analysis, the time courses of the expression levels of eight of the genes identified were found to include intrinsic induction time.
ISSN:0916-8451
1347-6947
1347-6947
DOI:10.1271/bbb.100565