Evaluation of a new cell separator for collection of peripheral blood CD34+ progenitor cells in pediatric patients

BACKGROUND: This study was conducted to evaluate the performance of the COM.TEC cell separator (Fresenius HemoCare GmbH) for collecting CD34+ cells in pediatric patients who were intended to have autologous peripheral blood progenitor cell transplantation, with respect to collection variables, predi...

Full description

Saved in:
Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 2011-02, Vol.51 (2), p.306-312
Main Authors: Kim, Suk-Ran, Choung, Hae-Kyoung, Kim, Dae-Won, Sung, Ki-Woong, Kang, Eun-Suk
Format: Article
Language:English
Subjects:
Adolescent
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Antigens, CD34 - analysis
Biological and medical sciences
Blood Cell Count
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Body Weight
Bone marrow, stem cells transplantation. Graft versus host reaction
Brain Neoplasms - blood
Child
Child, Preschool
Cytapheresis - instrumentation
Cytapheresis - methods
Female
Hematopoietic Stem Cell Mobilization
Hematopoietic Stem Cells - chemistry
Humans
Infant
Infant, Newborn
Leukapheresis - instrumentation
Leukapheresis - methods
Male
Medical sciences
Neuroblastoma - blood
Peripheral Blood Stem Cell Transplantation
Platelet Count
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:BACKGROUND: This study was conducted to evaluate the performance of the COM.TEC cell separator (Fresenius HemoCare GmbH) for collecting CD34+ cells in pediatric patients who were intended to have autologous peripheral blood progenitor cell transplantation, with respect to collection variables, prediction power of CD34+ cell yield, and influence on donors. STUDY DESIGN AND METHODS: A total of 26 pediatric solid tumor patients who received mobilization chemotherapy and granulocyte–colony‐stimulating factor underwent CD34+ cell collection (n = 96) using the COM.TEC auto mononuclear cell (MNC) program. Patients were divided into a neuroblastoma (NBL) group and a brain tumor group according to the intensity of prior chemotherapy regimens. The collection variables, cellular variables of leukapheresis products, and the peripheral blood cell counts of patients were compared with those acquired using the COBE Spectra (GambroBCT). The CD34+ cell collection efficiency (CE) and the percentage ratios of actual to predicted CD34+ cell yield indicating prediction power were analyzed. RESULTS: Using the COM.TEC auto MNC program, the processing rate was higher and the product volume was smaller (p 
ISSN:0041-1132
1537-2995
DOI:10.1111/j.1537-2995.2010.02864.x