Optical Nano-Constructs Composed of Genome-Depleted Brome Mosaic Virus Doped with a Near Infrared Chromophore for Potential Biomedical Applications

We have engineered an optical nanoconstruct composed of genome-depleted brome mosaic virus doped with indocyanine green (ICG), an FDA-approved near-infrared (NIR) chromophore. Constructs are highly monodispersed with standard deviation of ±3.8 nm from a mean diameter of 24.3 nm. They are physically...

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Bibliographic Details
Published in:ACS nano 2011-02, Vol.5 (2), p.1243-1252
Main Authors: Jung, Bongsu, Rao, Ayala L. N, Anvari, Bahman
Format: Article
Language:English
Subjects:
Bromovirus - chemistry
Bromovirus - genetics
Cell Line
Cell Survival - drug effects
Genome, Viral
Humans
Indocyanine Green - chemistry
Indocyanine Green - toxicity
Infrared Rays
Intracellular Space - metabolism
Molecular Imaging - methods
Optical Phenomena
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Summary:We have engineered an optical nanoconstruct composed of genome-depleted brome mosaic virus doped with indocyanine green (ICG), an FDA-approved near-infrared (NIR) chromophore. Constructs are highly monodispersed with standard deviation of ±3.8 nm from a mean diameter of 24.3 nm. They are physically stable and exhibit a high degree of optical stability at physiological temperature (37 °C). Using human bronchial epithelial cells, we demonstrate the effectiveness of the constructs for intracellular optical imaging in vitro, with greater than 90% cell viability after 3 h of incubation. These constructs may serve as a potentially nontoxic and multifunctional nanoplatform for site-specific deep-tissue optical imaging, and therapy of disease.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn1028696