In vitro anaerobic biofilms of human colonic microbiota

The human gastrointestinal tract hosts a complex community of microorganisms that grow as biofilms on the intestinal mucosa. These bacterial communities are not well characterized, although they are known to play an important role in human health. This study aimed to develop a model for culturing bi...

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Bibliographic Details
Published in:Journal of microbiological methods 2010-12, Vol.83 (3), p.296-301
Main Authors: Sproule-Willoughby, K.M., Stanton, M. Mark, Rioux, K.P., McKay, D.M., Buret, A.G., Ceri, H.
Format: Article
Language:English
Subjects:
Anaerobic bacteria
Anaerobiosis
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacterial Adhesion
Bacterial Physiological Phenomena
Biofilms
Biofilms - classification
Biological and medical sciences
Colon - chemistry
Colon - microbiology
Fundamental and applied biological sciences. Psychology
Humans
Intestinal microbiota
Intestinal Mucosa - chemistry
Intestinal Mucosa - microbiology
Metagenome
Microbiology
Microscopy, Confocal
Models, Biological
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Summary:The human gastrointestinal tract hosts a complex community of microorganisms that grow as biofilms on the intestinal mucosa. These bacterial communities are not well characterized, although they are known to play an important role in human health. This study aimed to develop a model for culturing biofilms (surface-adherent communities) of intestinal microbiota. The model utilizes adherent mucosal bacteria recovered from colonic biopsies to create multi-species biofilms. Culture on selective media and confocal microscopy indicated the biofilms were composed of a diverse community of bacteria. Molecular analyses confirmed that several phyla were represented in the model, and demonstrated stability of the community over 96h when cultured in the device. This model is novel in its use of a multi-species community of mucosal bacteria grown in a biofilm mode of growth.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2010.09.020