Zinc inhibits the mixed lymphocyte culture

The mixed lymphocyte culture (MLC) is an established clinical method for bone marrow transplantation, as it serves as an in vitro model for allogenic reaction and transplantation. We previously showed that cytokine release into the supernatant is a more specific and sensitive parameter for cross-rea...

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Bibliographic Details
Published in:Biological trace element research 2001-01, Vol.79 (1), p.15-22
Main Authors: Campo, C A, Wellinghausen, N, Faber, C, Fischer, A, Rink, L
Format: Article
Language:English
Subjects:
Bone marrow
Bone marrow transplantation
Bone Marrow Transplantation - methods
Cell culture
Cell Division
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Immune response
Immunosuppressive Agents - pharmacology
Interferon-gamma - biosynthesis
Lymphocytes
Lymphocytes - cytology
Lymphocytes - drug effects
Trace elements
Transplants & implants
Zinc
Zinc - metabolism
Zinc - pharmacology
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Summary:The mixed lymphocyte culture (MLC) is an established clinical method for bone marrow transplantation, as it serves as an in vitro model for allogenic reaction and transplantation. We previously showed that cytokine release into the supernatant is a more specific and sensitive parameter for cross-reactivity in the MLC than the common measurement of cell proliferation. Therefore we tried to find an inhibitor of the MLC in vitro with the least side effects in vivo, measuring interferon (IFN)-gamma as one of the most important cytokines in posttransplant medicine. Earlier studies showed that zinc is an important trace element for immune function with both stimulatory and inhibitory effects on immune cells. We found that slightly elevated zinc concentrations (three to four times the physiological level), which do not decrease T-cell proliferation in vitro nor produce immunosuppressive effects in vivo, suppress alloreactivity in the mixed lymphocyte culture. In this report we analyzed the mechanism whereby zinc influences the MLC to possibly find a nontoxic way of immunosuppression.
ISSN:0163-4984
0163-4984
1559-0720
DOI:10.1385/BTER:79:1:15