The effect of bioindicator preparation and storage on thermal resistance of Bacillus stearothermophilus spores

Paper strips inoculated with spores of Bacillus stearothermophilus ATCC 7953 were conventionally dried (lot 1) and lyophilized (lot 2); stored in defined environments of 32 and 86% relative humidity at 10, 25 and 33 degrees C for 210 d; and submitted to moist heat treatments at 121 degrees C. A sign...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2002, Vol.98-100 (1-9), p.525-538
Main Authors: VESSONI PENNA, Thereza Christina, ISHII, Marina, MACHOSHVILI, Irene Alexeevna, MARQUES, Marcelo
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Analysis of Variance
Applied microbiology
Bacillus stearothermophilus
Bacteria
Bacteriological Techniques - methods
Bacteriology
Bioindicators
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Geobacillus stearothermophilus - growth & development
Geobacillus stearothermophilus - physiology
Heat
Heat transfer
Heat treatment
Heat treatments
Hot Temperature
Humidity
Indicator species
Microbiology
Regression Analysis
Relative humidity
Spores
Spores, Bacterial
Sterilization methods
Thermal resistance
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Summary:Paper strips inoculated with spores of Bacillus stearothermophilus ATCC 7953 were conventionally dried (lot 1) and lyophilized (lot 2); stored in defined environments of 32 and 86% relative humidity at 10, 25 and 33 degrees C for 210 d; and submitted to moist heat treatments at 121 degrees C. A significant decrease in thermal resistance from initial starting levels was found for lyophilized bioindicators stored at 86% relative humidity. The respective average D121 degrees C values were 1.55+/-0.05 and 1.37+/-0.10 min for lyophilized bioindicators stored at 32 and 86% relative humidity; and 1.65+/-0.15 min and 1.57+/-0.11 min for dried bioindicators stored in the same environments.
ISSN:0273-2289
1559-0291
0273-2289
DOI:10.1385/ABAB:98-100:1-9:525