Gene mapping of 28S and 5S rDNA sites in the spined loach Cobitis taenia (Pisces, Cobitidae) from a diploid population and a diploid-tetraploid population

We compare the chromosomal 28S and 5S rDNA patterns of the spined loach C. taenia (2n = 48) from an exclusively diploid population and from a diploid-polyploid population using 28S and 5S rDNA probe preparation and labelling, and fluorescence in situ hybridization (FISH). The 5S rDNA was located in...

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Bibliographic Details
Published in:Genetica 2006-09, Vol.128 (1-3), p.71-79
Main Authors: Boroń, Alicja, Ozouf-Costaz, Catherine, Coutanceau, Jean-Pierre, Woroniecka, Katarzyna
Format: Article
Language:English
Subjects:
Animals
Chromosome Mapping
Chromosomes
Cobitidae
Cobitis
Cobitis taenia
Cypriniformes - genetics
Diploidy
DNA, Ribosomal - genetics
Female
Gene mapping
Genetics, Population
In Situ Hybridization, Fluorescence
Karyotypes
Male
Nucleolus Organizer Region
Poland
Polyploidy
Ribosomal DNA
RNA, Ribosomal, 28S - genetics
RNA, Ribosomal, 5S - genetics
Taenia
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Summary:We compare the chromosomal 28S and 5S rDNA patterns of the spined loach C. taenia (2n = 48) from an exclusively diploid population and from a diploid-polyploid population using 28S and 5S rDNA probe preparation and labelling, and fluorescence in situ hybridization (FISH). The 5S rDNA was located in two to three chromosome pairs, and separated from the 28S loci for the males and one female (F1) from the diploid population. Loaches from a diploid-polyploid population, and one female (F2) from the diploid population were characterized by at least one chromosome pair with 5S and 28S overlapping signals. The fishes differed mainly in their number of 28S rDNA loci, located on 3-6 chromosomes. All individuals from both populations were characterized by one acrocentric chromosome bearing a 28S rDNA signal on the telomeres of its long arm. The number of major ribosomal DNA in the karyotype of C. taenia by FISH was always higher than the number of Ag-NORs. Our data confirm the extensive polymorphism of NORs in both populations, as already has been observed in closely related Cobitis species, and less polymorphic 5S rDNA pattern. However, this preliminary result highlights the need for a wider scale study.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-005-5536-8