Spatiotemporal genetic differentiation of Cuban natural populations of the pink shrimp Farfantepenaeus notialis

• Published in: Genetica 2008-07, Vol.133 (3), p.283-294
• Main Authors: Robainas-Barcia, Aymée, Blanco, Gloria, Sánchez, José A, Monnerot, Monique, Solignac, Michel, García-Machado, Erik
• Format: Article
• Language: English
• Subjects: Alleles; Animal Genetics and Genomics; Animals; Base Sequence; Biomedical and Life Sciences; Cuba; Decapoda; Evolutionary Biology; Farfantepenaeus notialis; Genetic diversity; Genetic drift; Genetic structure; Genetic Variation - genetics; Gulfs; Human Genetics; Hurricanes; Life Sciences; Microbial Genetics and Genomics; Microsatellite Repeats; Natural populations; Penaeidae - genetics; Plant Genetics and Genomics; population structure; Temporal genetic variation; Time Factors
• ISSN: 0016-6707; 1573-6857
• DOI: 10.1007/s10709-007-9212-z

We analyzed the spatiotemporal genetic structure of Farfantepenaeus notialis populations using five microsatellites loci in order to understand the influence of natural events such as hurricanes on the genetic drift/migration balance as the main cause for the variation of allele frequencies over time. The results were compared with the previous ones obtained from allozymes and mtDNA. High and stable genetic diversity levels (He = 0.879 ± 0.0015) were found over eight years for the populations that inhabit the south Cuban platform, however significant changes of allele frequencies were detected over time. The F ST estimates, albeit low, revealed significant differences among populations inside the Ana Maria Gulf for 1995 but not for the 1999 and 2003 samples. The F ST, AMOVA and the genetic distance analysis revealed the instability of the genetic structure over time in accordance with allozymes results. The correspondence of the microsatellite results with those obtained from allozymes confirm the effects of migration enhanced by natural events as the main cause of the temporal variation of allele frequencies. The genetic drift effect was discarded through the evaluation of Ne and the M ratio, while natural selection effects were rejected because of the lowest probability of microsatellite loci being under selective pressures. The microsatellite data are also consistent with the results obtained with mtDNA in detecting significant and persistent genetic differences between the Gulfs of Ana María and Batabanó for the years 1995 and 2003.