Desulfosporomusa polytropa gen. nov., sp. nov., a novel sulfate-reducing bacterium from sediments of an oligotrophic lake

• Published in: Archives of microbiology 2004-10, Vol.182 (2-3), p.204-211
• Main Authors: Sass, Henrik, Overmann, Jörg, Rütters, Heike, Babenzien, Hans-Dietrich, Cypionka, Heribert
• Format: Article
• Language: English
• Subjects: Amino acids; Aromatic compounds; Bacteria; Bacterial Typing Techniques; Carbohydrates; Cytochromes b - metabolism; Desulfosporomusa polytropa; DNA, Bacterial - chemistry; DNA, Bacterial - isolation & purification; DNA, Ribosomal - chemistry; DNA, Ribosomal - isolation & purification; Endospore-Forming Bacteria - classification; Endospore-Forming Bacteria - isolation & purification; Ethanol; Ferric Compounds - metabolism; Firmicutes; Flagella; Fresh Water - microbiology; Freshwater; Genes, rRNA; Geologic Sediments - microbiology; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - isolation & purification; Microbiology; Molecular Sequence Data; Movement; Oligotrophic lakes; Organic acids; Oxidation-Reduction; Oxidoreductases Acting on Sulfur Group Donors - metabolism; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology; Sporomusa; Sulfate reduction; Sulfates; Sulfates - metabolism; Sulfite Reductase (NADPH); Sulfur; Temperature; Veillonellaceae - classification; Veillonellaceae - cytology; Veillonellaceae - isolation & purification; Veillonellaceae - physiology; Water Microbiology
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-004-0703-3

Five strains of sulfate-reducing bacteria were isolated from the highest positive dilutions of a most probable number (MPN) series supplemented with lactate and inoculated with sediments from the oligotrophic Lake Stechlin. The isolates were endospore-forming and were motile by means of laterally inserted flagella. They stained Gram-negative and contained b-type cytochromes. CO difference spectra indicated the presence of P582 as a sulfite reductase. Phylogenetic analyses of the 16S rDNA sequences revealed that the isolates were very closely affiliated with the genus Sporomusa. However, sulfate and amorphous Fe(OH)(3), but not sulfite, elemental sulfur, MnO(2), or nitrate were used as terminal electron acceptors. Homoacetogenic growth was found with H(2)/CO(2) gas mixture, formate, methanol, ethanol, and methoxylated aromatic compounds. The strains grew autotrophically with H(2) plus CO(2) in the presence or absence of sulfate. Formate, butyrate, several alcohols, organic acids, carbohydrates, some amino acids, choline, and betaine were also utilized as substrates. The growth yield with lactate and sulfate as substrate was 7.0 g dry mass/mol lactate and thus two times higher than in sulfate-free fermenting cultures. All isolates were able to grow in a temperature range of 4-37 degrees C. Physiologically and by the presence of a Gram-negative cell wall, the new isolates resemble known Desulfosporosinus species. However, phylogenetically they are affiliated with the Gram-negative genus Sporomusa belonging to the Selenomonas subgroup of the Firmicutes. Therefore, the new isolates reveal a new phylogenetic lineage of sulfate-reducing bacteria. A new genus and species, Desulfosporomusa polytropa gen. nov., sp. nov. is proposed.