The protruding domain of the coat protein of Melon necrotic spot virus is involved in compatibility with and transmission by the fungal vector Olpidium bornovanus

Abstract The Chi and W strains of Melon necrotic spot virus (MNSV) are efficiently transmitted by isolates Y1 and NW1, respectively, of the fungal vector Olpidium bornovanus . Analysis of chimeric viruses constructed by switching the coat protein (CP) gene between the two strains unveiled the involv...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2010-06, Vol.402 (1), p.129-134
Main Authors: Ohki, Takehiro, Akita, Fusamichi, Mochizuki, Tomofumi, Kanda, Ayami, Sasaya, Takahide, Tsuda, Shinya
Format: Article
Language:English
Subjects:
Capsid Proteins - chemistry
Capsid Proteins - genetics
Capsid Proteins - physiology
Carmovirus - genetics
Carmovirus - physiology
Chimera
Chytridiomycota - virology
Coat protein
Cucurbitaceae - virology
Fungal transmission
Infectious Disease
Melon necrotic spot virus
Models, Molecular
Olpidium
Olpidium bornovanus
Protein Structure, Tertiary
Recombination, Genetic
Spores, Fungal - virology
Virus Attachment
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Summary:Abstract The Chi and W strains of Melon necrotic spot virus (MNSV) are efficiently transmitted by isolates Y1 and NW1, respectively, of the fungal vector Olpidium bornovanus . Analysis of chimeric viruses constructed by switching the coat protein (CP) gene between the two strains unveiled the involvement of the CP in the attachment of MNSV to zoospores of a compatible isolate of O. bornovanus and in the fungal transmission of the virus. Furthermore, analysis of the chimeric virus based on the Chi strain with the protruding domain of the CP from strain W suggested the involvement of the domain in compatibility with zoospore. Comparison of the three-dimensional structures between the CP of the two MNSV strains showed that many of the differences in these amino acid residues are present on the surface of the virus particles, suggesting that these affects the recognition of fungal vectors by the virus.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2010.03.020