Salicylic acid promotes seed germination under high salinity by modulating antioxidant activity in Arabidopsis

• Published in: The New phytologist 2010-10, Vol.188 (2), p.626-637
• Main Authors: Lee, Sangmin, Kim, Sang-Gyu, Park, Chung-Mo
• Format: Article
• Language: English
• Subjects: antioxidant; Antioxidants; Antioxidants - metabolism; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - enzymology; Arabidopsis - growth & development; Biodegradation; Catechol; Catechols; Catechols - pharmacology; Genotypes; Germination; Germination - drug effects; Growth conditions; Hydrogen; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Hydroxylase; Imbibition; Intramolecular Transferases - metabolism; Molecular modelling; Osmosis - drug effects; Peroxidase - metabolism; Peroxides; Plant growth; Plants; Salicylate hydroxylase; Salicylic acid; salicylic acid (SA); Salicylic Acid - pharmacology; Salinity; Salinity effects; salt stress; Seed germination; Seeds; Seeds - drug effects; Seeds - enzymology; Seeds - growth & development; Stress, Physiological - drug effects; Transgenic plants
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/j.1469-8137.2010.03378.x

Findings regarding the role of salicylic acid (SA) in seed germination are somewhat variable, depending on the plant genotypes and experimental conditions used, and thus the molecular mechanisms underlying SA regulation of germination are still unclear. Here, we report that physiological concentrations of SA promote germination under high salinity by modulating antioxidant activity in Arabidopsis. Germination of SA induction deficient 2 (sid2) seeds was hypersensitive to high salinity. While the inhibitory effect of high salinity was exaggerated in the presence of higher concentrations of SA (> 100 μM), it was significantly reduced in the presence of lower concentrations of SA (< 50 μM). Under high salinity, the endogenous contents of H₂O₂ were elevated in wild-type and sid2 seeds but reduced to original concentrations after treatment with 1 μM SA. Germination of NahG transgenic plants was influenced to a lesser degree by high salinity (NahG is a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol). We found that catechol, an SA degradation product accumulated in the transgenic plants, acts as an antioxidant that compromises the inhibitory effects of high salinity. Our observations indicate that, although SA is not essential for germination under normal growth conditions, it plays a promotive role in seed germination under high salinity by reducing oxidative damage.