Evaluation of Plasmodium vivax ELISA for the blood screen

Plasmodium vivax malaria is the indigenous strain in the Republic of Korea (ROK). Plasmodium vivax can be transmitted through the transfusions of various blood components, which became a severe problem with the safety of blood transfusions and blood‐related products in ROK. We evaluated a P. vivax‐s...

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Bibliographic Details
Published in:Tropical medicine & international health 2010-12, Vol.15 (12), p.1436-1441
Main Authors: Nam, Myung‐Hyun, Kim, Jang Su, Cho, Chi Hyun, Han, Eun Taek, Lee, Won Ja, Lee, Hee Kyung, An, Seong Soo A, Lim, Chae Seung, Lee, Kap No
Format: Article
Language:English
Subjects:
Antibodies, Protozoan - blood
antibody test
Biological and medical sciences
Blood Donors
Blood tests
Blood-Borne Pathogens - isolation & purification
ELISA
Endemic Diseases
Enzyme-Linked Immunosorbent Assay - methods
General aspects
Humans
Malaria
Malaria, Vivax - diagnosis
Malaria, Vivax - epidemiology
Malaria, Vivax - transmission
Mass Screening - methods
Medical sciences
Parasitemia - diagnosis
Pathogens
Plasmodium vivax
Plasmodium vivax - immunology
Republic of Korea
Republic of Korea - epidemiology
Sensitivity and Specificity
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Summary:Plasmodium vivax malaria is the indigenous strain in the Republic of Korea (ROK). Plasmodium vivax can be transmitted through the transfusions of various blood components, which became a severe problem with the safety of blood transfusions and blood‐related products in ROK. We evaluated a P. vivax‐specific enzyme‐linked immunosorbent assay (Genedia Malaria Ab ELISA 2.0, Green Cross, ROK) with blood samples from four groups: 251 samples from P. vivax‐infected patients, 39 samples from post‐treatment patients upon follow‐up, 200 samples from healthy volunteers and 421 samples from domestic travellers to and from high endemic areas of ROK. The positive cases from the ELISA test were confirmed by both Giemsa microscopic and polymerase chain reaction methods. The clinical sensitivity and specificity of detecting P. vivax with ELISA test were 94.4% and 99.0%, respectively. Thirteen of 421 domestic travellers (3.0%) to endemic areas tested positive. The results indicate the effectiveness of detecting antibodies against P. vivax in blood with Genedia Malaria Ab ELISA 2.0 test in a large blood screen setting.
ISSN:1360-2276
1365-3156
DOI:10.1111/j.1365-3156.2010.02657.x