Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation

In vitro ovulation of mature medaka ovarian follicles was inhibited by inhibitors of cyclooxygenase (COX) or by an antagonist of the prostaglandin E2 receptor (EP). Of the three medaka COX genes, ptgs2 was most dominantly expressed in the fish ovary. The ptgs2 transcript was detected in all sizes of...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2011-01, Vol.332 (1-2), p.67-77
Main Authors: Fujimori, Chika, Ogiwara, Katsueki, Hagiwara, Akane, Rajapakse, Sanath, Kimura, Atsushi, Takahashi, Takayuki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cellular
Cyclooxygenase 2 - genetics
Cyclooxygenase 2 - metabolism
Cyclooxygenase 2 Inhibitors - metabolism
Cyclooxygenase 2 Inhibitors - pharmacology
Cyclooxygenase-2
Endocrinology
EP receptor
Female
Fish
Freshwater
Gene expression
Genes
In vitro testing
Indomethacin - metabolism
Indomethacin - pharmacology
Inhibitors
Isoenzymes - genetics
Isoenzymes - metabolism
Medaka
Molecular Sequence Data
Oryzias - anatomy & histology
Oryzias - genetics
Oryzias - metabolism
Ovaries
Ovary - cytology
Ovary - metabolism
Ovulation
Ovulation - drug effects
Ovulation - physiology
Prostaglandin E2
Prostaglandins
Receptors
Receptors, Prostaglandin E, EP4 Subtype - antagonists & inhibitors
Receptors, Prostaglandin E, EP4 Subtype - genetics
Receptors, Prostaglandin E, EP4 Subtype - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Alignment
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Summary:In vitro ovulation of mature medaka ovarian follicles was inhibited by inhibitors of cyclooxygenase (COX) or by an antagonist of the prostaglandin E2 receptor (EP). Of the three medaka COX genes, ptgs2 was most dominantly expressed in the fish ovary. The ptgs2 transcript was detected in all sizes of growing follicles. In a 24-h spawning cycle, large-sized follicles contained ptgs2 mRNA at a fairly constant level. The levels of COX enzyme activity and prostaglandin E2 were also constant in the large-sized follicles during the spawning cycle. The expression of prostaglandin E2 receptor EP4b (ptger4b) mRNA was drastically upregulated in the large-sized follicles as the ovulation time approached. The current results indicate that prostaglandin E2, which might be produced by COX-2, is involved in the ovulation of medaka, and that EP4b is likely the receptor responsible for exerting the action of prostaglandin E2 in the process.
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2010.09.015