13C Direct-Detection Biomolecular NMR Spectroscopy in Living Cells

• Published in: Angewandte Chemie International Edition 2011-03, Vol.50 (10), p.2339-2341
• Main Authors: Bertini, Ivano, Felli, Isabella C., Gonnelli, Leonardo, Kumar M. V., Vasantha, Pierattelli, Roberta
• Format: Article
• Language: English
• Subjects: alpha-Synuclein - chemistry; alpha-Synuclein - genetics; alpha-Synuclein - metabolism; biological cells; biomolecules; Carbon Isotopes - chemistry; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cation Transport Proteins - chemistry; Cation Transport Proteins - genetics; Cation Transport Proteins - metabolism; Escherichia coli - metabolism; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - metabolism; isotopic labeling; Molecular Chaperones - chemistry; Molecular Chaperones - genetics; Molecular Chaperones - metabolism; NMR spectroscopy; Nuclear Magnetic Resonance, Biomolecular; Protein Unfolding; proteins; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.201006636

A direct response: Exclusively heteronuclear 13C direct‐detection 2D NMR experiments are performed on 13C,15N‐enriched proteins in E. coli cells. Unfolded proteins or protein fragments provide well‐resolved carbonyl detection (see picture), whereas folded proteins or structured motifs do not give any detectable spectrum.