Quality control of a herbal medicinal preparation using high-performance liquid chromatographic and capillary electrophoretic methods

Two methods based on high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) were developed for the quality control of “samgiumgagambang” (SGMX), a new herbal medicinal preparation containing 14 herbs. Nine components from SGMX were selected as markers: 5-hydroxymethylfurald...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2011-04, Vol.55 (1), p.206-210
Main Authors: Chen, Jianbo, Zhu, Hongmei, Chu, Van Men, Jang, Yu Seon, Son, Jin Young, Kim, Young Ho, Son, Chang Gyu, Seol, In Chan, Kang, Jong Seong
Format: Article
Language:English
Subjects:
acetonitrile
Analysis
Analytical, structural and metabolic biochemistry
berberine
Biological and medical sciences
Calibration
Capillary electrophoresis
Cardiovascular Diseases - drug therapy
Cardiovascular Diseases - prevention & control
chlorogenic acid
Chromatography, High Pressure Liquid
ecdysterone
Electrophoresis, Capillary
formic acid
Fundamental and applied biological sciences. Psychology
General pharmacology
glycyrrhizin
herbs
High performance liquid chromatography
Limit of Detection
luteolin
Marker component
Medical sciences
methanol
Pharmacology. Drug treatments
Phytotherapy
Plant Extracts - chemistry
Quality Control
Reproducibility of Results
Republic of Korea
Samgiumgagambang
silica
Technology, Pharmaceutical
Time Factors
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Summary:Two methods based on high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) were developed for the quality control of “samgiumgagambang” (SGMX), a new herbal medicinal preparation containing 14 herbs. Nine components from SGMX were selected as markers: 5-hydroxymethylfuraldehyde, geniposidic acid, chlorogenic acid, paeoniflorin, 20-hydroxyecdysone, coptisine, berberine, luteolin, and glycyrrhizic acid. The markers were identified and analyzed using HPLC coupled with a UV-diode-array detector and monitored at 250 nm with a gradient elution of acetonitrile and water containing formic acid on a C 18 analytical column or using CE with a 70 mM borate buffer (pH 9.5) containing 10% methanol on a 60-cm fused silica capillary monitored at 230 nm. The marker components in SGMX were well separated using both methods and were readily determined within 60 min using HPLC or 13 min using CE with good precision and accuracy.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2010.12.022