Fluorescence resonance energy transfer from tryptophan to folic acid in micellar media and deionised water

The fluorescence resonance energy transfer (FRET) from tryptophan (Trp) to folic acid (FA) in aqueous sodiumdodecyl sulphate, cetyltrimethyl ammonium bromide, and Brij-35 as well as deionised water was investigated using steady state and time resolved fluorescence techniques. The data obtained from...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2011-04, Vol.103 (1), p.16-21
Main Authors: Mote, U.S., Patil, S.R., Bhosale, S.H., Han, S.H., Kolekar, G.B.
Format: Article
Language:English
Subjects:
Absorption
binding sites
cetyltrimethylammonium bromide
energy transfer
fluorescence
Fluorescence quenching
Fluorescence Resonance Energy Transfer
Fluorescence resonance energy transfer (FRET)
Folic acid
Folic Acid - chemistry
Foster theory
hydrogen bonding
hydrophobicity
Micelles
photochemistry
sodium dodecyl sulfate
spectral analysis
Stern–Volmer plot
sulfates
sulphates
Surface-Active Agents - chemistry
Temperature
Thermodynamics
Time Factors
Tryptophan
Tryptophan - chemistry
Water - chemistry
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Summary:The fluorescence resonance energy transfer (FRET) from tryptophan (Trp) to folic acid (FA) in aqueous sodiumdodecyl sulphate, cetyltrimethyl ammonium bromide, and Brij-35 as well as deionised water was investigated using steady state and time resolved fluorescence techniques. The data obtained from steady state fluorescence spectral studies and time resolved measurement indicated that the FRET from Trp to FA occurred most effectively in aqueous sodium dodecyl sulphate micellar solutions. The distance between Trp and FA were evaluated. Binding constant, number of binding sites and thermodynamic parameters were determined for Trp–FA interactions in deionised water. The values of the thermodynamic parameters suggest that the hydrophobic forces and hydrogen bonding are the key interacting forces between Trp–FA interaction.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2011.01.006