Extraction of MS2 Phage RNA from Upper Respiratory Tract Specimens by Use of Flat Glass Devices

The isolation of pure nucleic acids from clinical samples is a crucial step in the molecular diagnosis of viral infections by nucleic acid testing (NAT). In this study, novel flat glass devices (cards) were demonstrated to support the rapid and efficient extraction of nucleic acids from upper respir...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2011-03, Vol.49 (3), p.1010-1016
Main Authors: Nanassy, Oliver Z, Haydock, Paul, Beck, Nicola, Barker, Lynn M, Hargrave, Perry, Gestwick, Daniel, Lindsey, Wesley C, Reed, Michael W, Meschke, J. Scott
Format: Article
Language:English
Subjects:
Automation - methods
Biological and medical sciences
Bodily Secretions - virology
Equipment and Supplies
Fundamental and applied biological sciences. Psychology
Glass
Humans
Levivirus - genetics
Levivirus - isolation & purification
Microbiology
Miscellaneous
Respiratory System
RNA, Viral - genetics
RNA, Viral - isolation & purification
Sensitivity and Specificity
Virology
Virology - methods
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Summary:The isolation of pure nucleic acids from clinical samples is a crucial step in the molecular diagnosis of viral infections by nucleic acid testing (NAT). In this study, novel flat glass devices (cards) were demonstrated to support the rapid and efficient extraction of nucleic acids from upper respiratory tract specimens (nasal washes and swabs). The performance of the nucleic acid extraction cards was directly compared to an existing standardized and automated platform for viral extraction from these types of specimens. The flowthrough card method improved the speed of nucleic acid purification and accommodated larger sample volumes in extraction of bacteriophage MS2 RNA from the various specimen matrices. The dynamic range and estimated sensitivity of the card extraction method for reverse transcriptase quantitative real-time PCR (RT-qPCR)-based detection approximate those of the standardized magnetic glass bead extraction method used in this study.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.01132-10