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Extraction of MS2 Phage RNA from Upper Respiratory Tract Specimens by Use of Flat Glass Devices
The isolation of pure nucleic acids from clinical samples is a crucial step in the molecular diagnosis of viral infections by nucleic acid testing (NAT). In this study, novel flat glass devices (cards) were demonstrated to support the rapid and efficient extraction of nucleic acids from upper respir...
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Published in: | Journal of Clinical Microbiology 2011-03, Vol.49 (3), p.1010-1016 |
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container_title | Journal of Clinical Microbiology |
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creator | Nanassy, Oliver Z Haydock, Paul Beck, Nicola Barker, Lynn M Hargrave, Perry Gestwick, Daniel Lindsey, Wesley C Reed, Michael W Meschke, J. Scott |
description | The isolation of pure nucleic acids from clinical samples is a crucial step in the molecular diagnosis of viral infections by nucleic acid testing (NAT). In this study, novel flat glass devices (cards) were demonstrated to support the rapid and efficient extraction of nucleic acids from upper respiratory tract specimens (nasal washes and swabs). The performance of the nucleic acid extraction cards was directly compared to an existing standardized and automated platform for viral extraction from these types of specimens. The flowthrough card method improved the speed of nucleic acid purification and accommodated larger sample volumes in extraction of bacteriophage MS2 RNA from the various specimen matrices. The dynamic range and estimated sensitivity of the card extraction method for reverse transcriptase quantitative real-time PCR (RT-qPCR)-based detection approximate those of the standardized magnetic glass bead extraction method used in this study. |
doi_str_mv | 10.1128/JCM.01132-10 |
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The dynamic range and estimated sensitivity of the card extraction method for reverse transcriptase quantitative real-time PCR (RT-qPCR)-based detection approximate those of the standardized magnetic glass bead extraction method used in this study.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.01132-10</identifier><identifier>PMID: 21191051</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Automation - methods ; Biological and medical sciences ; Bodily Secretions - virology ; Equipment and Supplies ; Fundamental and applied biological sciences. 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Scott</creatorcontrib><title>Extraction of MS2 Phage RNA from Upper Respiratory Tract Specimens by Use of Flat Glass Devices</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The isolation of pure nucleic acids from clinical samples is a crucial step in the molecular diagnosis of viral infections by nucleic acid testing (NAT). In this study, novel flat glass devices (cards) were demonstrated to support the rapid and efficient extraction of nucleic acids from upper respiratory tract specimens (nasal washes and swabs). The performance of the nucleic acid extraction cards was directly compared to an existing standardized and automated platform for viral extraction from these types of specimens. The flowthrough card method improved the speed of nucleic acid purification and accommodated larger sample volumes in extraction of bacteriophage MS2 RNA from the various specimen matrices. The dynamic range and estimated sensitivity of the card extraction method for reverse transcriptase quantitative real-time PCR (RT-qPCR)-based detection approximate those of the standardized magnetic glass bead extraction method used in this study.</description><subject>Automation - methods</subject><subject>Biological and medical sciences</subject><subject>Bodily Secretions - virology</subject><subject>Equipment and Supplies</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glass</subject><subject>Humans</subject><subject>Levivirus - genetics</subject><subject>Levivirus - isolation & purification</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Respiratory System</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>Sensitivity and Specificity</subject><subject>Virology</subject><subject>Virology - methods</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkU1vEzEQhi0EoqFw4wzmgHrplvHHeteXSlVoC6gF1DQSN8tx7MTV7nqxN6X593WaUODEyZLnmXdm9CD0msARIbT-8GV8eQSEMFoQeIJGBGRdCAE_nqIRgCyLXKr20IuUbgAI52X5HO1RQiSBkoyQOr0bojaDDx0ODl9OKP6-1AuLr76eYBdDi6d9byO-sqn3UQ8hrvH1pgFPemt8a7uEZ2s8TXbTftboAZ83OiX80d56Y9NL9MzpJtlXu3cfTc9Or8efiotv55_HJxeF4bIciprPuNAzEDCnnDFacUEc5YZrwTijNals5ZwDWpXW8NrOSxBGG0szqWFes310vM3tV7PWzo3t8lmN6qNvdVyroL36t9L5pVqEW8VAVBVUOeBgFxDDz5VNg2p9MrZpdGfDKikJnItKCvgvWZclBSo5yeThljQxpBSte9yHgNrIU1meepCXfzL-5u8bHuHftjLwfgfoZHTjou6MT384JkmVDWfu3ZZb-sXyl49W6dSqG9MqLhXLox6Gvd0yTgelFzHnTCcUCAMieS2ZYPdwvbR9</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Nanassy, Oliver Z</creator><creator>Haydock, Paul</creator><creator>Beck, Nicola</creator><creator>Barker, Lynn M</creator><creator>Hargrave, Perry</creator><creator>Gestwick, Daniel</creator><creator>Lindsey, Wesley C</creator><creator>Reed, Michael W</creator><creator>Meschke, J. 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subjects | Automation - methods Biological and medical sciences Bodily Secretions - virology Equipment and Supplies Fundamental and applied biological sciences. Psychology Glass Humans Levivirus - genetics Levivirus - isolation & purification Microbiology Miscellaneous Respiratory System RNA, Viral - genetics RNA, Viral - isolation & purification Sensitivity and Specificity Virology Virology - methods |
title | Extraction of MS2 Phage RNA from Upper Respiratory Tract Specimens by Use of Flat Glass Devices |
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